Zhang W, McElroy D, Wu R
Cornell University, Ithaca, New York 14853.
Plant Cell. 1991 Nov;3(11):1155-65. doi: 10.1105/tpc.3.11.1155.
The 5' region of the rice actin 1 gene (Act1) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants. To determine the pattern and level of rice Act1 5' region activity, transgenic rice plants containing the Act1 5' region fused to a bacterial beta-glucuronidase (Gus) coding sequence were generated. Two independent clonal lines of transgenic rice plants were analyzed in detail. Quantitative analysis showed that tissue from these transgenic rice plants have a level of GUS protein that represents as much as 3% of total soluble protein. We were able to demonstrate that Act1-Gus gene expression is constitutive throughout the sporophytic and gametophytic tissues of these transgenic rice plants. Plants from one transgenic line were analyzed for the segregation of GUS activity in pollen by in situ histochemical staining, and the inheritance and stability of Act1-Gus expression were assayed in subsequently derived progeny plants.
水稻肌动蛋白1基因（Act1）的5'区域已被开发为转基因水稻植株中外源基因表达的有效调控元件。为了确定水稻Act1 5'区域活性的模式和水平，构建了含有与细菌β-葡萄糖醛酸酶（Gus）编码序列融合的Act1 5'区域的转基因水稻植株。对两个独立的转基因水稻克隆系进行了详细分析。定量分析表明，这些转基因水稻植株的组织中GUS蛋白水平高达总可溶性蛋白的3%。我们能够证明，Act1-Gus基因在这些转基因水稻植株的孢子体和配子体组织中均组成型表达。通过原位组织化学染色分析了一个转基因系植株花粉中GUS活性的分离情况，并在随后衍生的后代植株中检测了Act1-Gus表达的遗传和稳定性。
