Park Min-Ju, Kim Myung Kyum, Kim Ho-Bin, Im Wan-Taek, Yi Tae-Hoo, Kim Se-Young, Soung Nak-Kyun, Yang Deok-Chun
Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):429-33. doi: 10.1099/ijs.0.65226-0.
Strain Gsoil 259(T), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain Gsoil 259(T) belonged to the genus Microbacterium and was closely related to Microbacterium arborescens IFO 3750(T) (98.5 %) and Microbacterium imperiale IFO 12610(T) (97.9 %). However, it has low values for DNA-DNA relatedness with the above strains (20.7 and 17.5 %, respectively). Strain Gsoil 259(T) possessed chemotaxonomic markers that were consistent with classification in the genus Microbacterium, i.e. MK-11 and MK-12 were the major menaquinones and anteiso-C(17 : 0), anteiso-C(15 : 0) and iso-C(16 : 0) were the predominant cellular fatty acids. The DNA G+C content was 69.4 mol%. The cell-wall sugar was rhamnose and the diamino acid in the cell-wall peptidoglycan was ornithine. On the basis of data from this polyphasic study, strain Gsoil 259(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium ginsengisoli sp. nov. is proposed. The type strain is Gsoil 259(T) (=KCTC 19189(T) =DSM 18659(T)).
菌株Gsoil 259(T)是一种产β-葡萄糖苷酶的细菌，从韩国人参田的土壤样品中分离得到，并对其进行了特征分析以确定其分类地位。细胞为革兰氏阳性、异养、严格需氧、无运动性的短杆菌。16S rRNA基因序列分析表明，菌株Gsoil 259(T)属于微杆菌属，与树木微杆菌IFO 3750(T)（98.5%）和帝王微杆菌IFO 12610(T)（97.9%）密切相关。然而，它与上述菌株的DNA-DNA相关性值较低（分别为20.7%和17.5%）。菌株Gsoil 259(T)具有与微杆菌属分类一致的化学分类标记，即MK-11和MK-12是主要的甲基萘醌，anteiso-C(17 : 0)、anteiso-C(15 : 0)和iso-C(16 : 0)是主要的细胞脂肪酸。DNA G+C含量为69.4 mol%。细胞壁糖为鼠李糖，细胞壁肽聚糖中的二氨基酸为鸟氨酸。基于这一多相研究的数据，菌株Gsoil 259(T)代表微杆菌属的一个新物种，为此提出了人参微杆菌新种Microbacterium ginsengisoli sp. nov.。模式菌株为Gsoil 259(T)（=KCTC 19189(T) =DSM 18659(T)）。
