Edwardson P A, Walker M, Gardner R S, Jacques E
Conva Tec Product Research Laboratory, Techbase 2B, Deeside, Clwyd, UK.
J Pharm Biomed Anal. 1991;9(10-12):1089-94. doi: 10.1016/0731-7085(91)80049-f.
Measurement of the water content of stratum corneum plays an important role in physiological and therapeutic inquiries in dermatology. There are many techniques available for non-invasive determination of skin hydration such as measurement of electrical, mechanical, thermal and spectroscopic properties of the skin. Most techniques, however, suffer from the fact that they do not employ a direct measurement of water content rather a property caused by skin hydration. Recently, Potts et al., (Arch. Derm. Res. 277, 489-495, 1985) developed an FT-IR method for the determination of water content of the skin both in vitro and in vivo. The method employed attenuated total reflectance infrared (ATR-IR) to measure a weak O-H stretch formed by the presence of water at 2100 cm-1. This absorbance is distant from interferences due to skin and most topically applied substances and therefore may be used in the quantitation of skin water content (hydration). This report describes the use of this technique in an investigation into the effect of occlusion on the water content of the skin. Method development and validation employing an in vitro system is also discussed.
角质层含水量的测定在皮肤病学的生理学和治疗学研究中起着重要作用。有许多技术可用于无创测定皮肤水合作用，如测量皮肤的电学、力学、热学和光谱学性质。然而，大多数技术存在这样一个问题，即它们并非直接测量含水量，而是测量由皮肤水合作用引起的一种性质。最近，波茨等人（《皮肤病学研究文献》277卷，489 - 495页，1985年）开发了一种傅里叶变换红外光谱法（FT - IR），用于在体外和体内测定皮肤的含水量。该方法采用衰减全反射红外光谱（ATR - IR）来测量在2100厘米-1处由水的存在形成的微弱O - H伸缩振动。这种吸光度远离皮肤和大多数局部应用物质产生的干扰，因此可用于定量皮肤含水量（水合作用）。本报告描述了该技术在一项关于封闭对皮肤含水量影响的研究中的应用。还讨论了采用体外系统进行方法开发和验证的情况。
