Division of Nephrology and Hypertension, Department of Internal Medicine, Katsushika Medical Center, Jikei University School of Medicine, 6-41-2 Katsushika-ku, Tokyo, 125-8506, Japan.
Division of Nephrology and Hypertension, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Clin Exp Nephrol. 2019 Jul;23(7):928-938. doi: 10.1007/s10157-019-01724-7. Epub 2019 Mar 16.
A decrease in absolute numbers (abs.) of circulating dendritic cells (DCs) and recruitment into target organs has been reported, but whether the level of proteinuria associates with circulating DC abs. has not been clarified.
We conducted a cross-sectional study of 210 patients with kidney disease aged 21-96 years who were admitted to our hospital for kidney biopsy in 2007-2010. For accuracy, the level of proteinuria was thoroughly measured by 24-h urine collection from patients in their admitted condition. The abs. of total DCs (tDCs), myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) was measured by three-color fluorescence-activated cell sorting (FACS). Patients were divided into four groups based upon the quartile of each DC abs. and one-way ANOVA, and multivariable-adjusted regression analyses were performed.
Quantile analysis showed that the level of daily proteinuria decreased with increasing blood mDC abs., with mean proteinuria levels (g/day) of 2.45, 1.68, 1.68, 1.10 for those in mDC abs. quartiles ≤ 445, < 686, < 907, ≥ 907 cells/10 µL (p = 0.0277), respectively. Multivariate-adjusted regression analysis revealed that the mDC abs. was negatively associated with proteinuria (95% CI - 57.0 to - 8.5) and positively associated with male gender (95% CI 66.2-250.5). Independent associations were also shown between pDCs abs. and estimated glomerular filtration rate (eGFR) (95% CI 0.14-2.67) and C-reactive protein (95% CI - 49.4 to - 9.9) and between tDCs abs. and male gender (95% CI 54.5-253.6) and C-reactive protein (95% CI - 80.5 to - 13.4).
We first reported that circulating mDC abs. has a negative association with the level of proteinuria.
据报道，循环树突状细胞（DC）的绝对数量减少（abs.）和募集到靶器官，但蛋白尿的水平是否与循环 DC abs.有关尚未阐明。
我们对 2007 年至 2010 年间因肾脏活检而入院的 210 名年龄在 21-96 岁的肾脏疾病患者进行了横断面研究。为了准确性，通过患者入院时的 24 小时尿液收集彻底测量蛋白尿的水平。通过三色荧光激活细胞分选（FACS）测量总 DC（tDC）、髓样 DC（mDC）和浆细胞样 DC（pDC）的 abs.。根据每个 DC abs.的四分位数将患者分为四组，进行单因素方差分析，并进行多变量调整回归分析。
定量分析显示，每日蛋白尿水平随血液 mDC abs.的增加而降低，mDC abs.四分位数≤445、<686、<907、≥907 细胞/10 µL 的患者平均蛋白尿水平（g/天）分别为 2.45、1.68、1.68、1.10（p=0.0277）。多变量调整回归分析显示，mDC abs.与蛋白尿呈负相关（95%CI-57.0 至-8.5），与男性呈正相关（95%CI66.2-250.5）。还显示 pDCs abs.与估计肾小球滤过率（eGFR）（95%CI0.14-2.67）和 C 反应蛋白（95%CI-49.4 至-9.9）之间存在独立关联，以及 tDCs abs.与男性（95%CI54.5-253.6）和 C 反应蛋白（95%CI-80.5 至-13.4）之间存在独立关联。
我们首次报道循环 mDC abs.与蛋白尿水平呈负相关。
