Makino O, Saito H, Ando T
Mol Gen Genet. 1980;179(3):463-8. doi: 10.1007/BF00271735.
Bacillus amyloliquefaciens N produces two restriction enzymes, BamNI and BamNx. Subtilis-phage phi 1 is strongly restricted by BamNx. We isolated phi 1 rH, a mutant of phage phi 1, which overcame the BamNx-restriction by producing inhibitor. This inhibitor inactivated BamNx specifically and reversibly. The inhibitor directly interacted with BamNx and the inactivation might be the result of formation of a binary complex. The inhibitory activity was sensitive to treatment with trypsin. The molecular weight of the inhibitor protein was estimated to be approximately 20,000 daltons by gel filtration.
解淀粉芽孢杆菌N产生两种限制酶，BamNI和BamNx。枯草芽孢杆菌噬菌体phi 1受到BamNx的强烈限制。我们分离出了噬菌体phi 1的一个突变体phi 1 rH，它通过产生抑制剂克服了BamNx的限制。这种抑制剂特异性且可逆地使BamNx失活。该抑制剂直接与BamNx相互作用，失活可能是形成二元复合物的结果。抑制活性对胰蛋白酶处理敏感。通过凝胶过滤估计抑制剂蛋白的分子量约为20,000道尔顿。
