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用枯草芽孢杆菌N无细胞提取物对噬菌体phi - 105c DNA进行体外修饰和限制

In vitro modification and restriction of phage phi-105c DNA with Bacillus subtilis N cell-free extract.

作者信息

Shibata T, Ando T

出版信息

Mol Gen Genet. 1975 Jul 10;138(4):269-79. doi: 10.1007/BF00264796.

DOI:10.1007/BF00264796
PMID:807794
Abstract

The enzymes involved in host-controlled modification and restriction by Bacillus subtilis strain N were detected in cell free extracts. In the presenct of Mg2+ the N-specific endonucleases cleaved unmodified DNA but did not attack phi-105C. N DNA carrying N-specific modification. The restriction endonuclease required neither SAM nor ATP for its activity. The N-specific modification enzyme was active only in the presence of SAM, indicating that modification in this syteem is a methylation of DNA.

摘要

在无细胞提取物中检测到了枯草芽孢杆菌N菌株中参与宿主控制修饰和限制作用的酶。在Mg2+存在的情况下，N特异性核酸内切酶可切割未修饰的DNA，但不攻击携带N特异性修饰的噬菌体105C的N DNA。该限制性核酸内切酶的活性既不需要SAM也不需要ATP。N特异性修饰酶仅在SAM存在时才有活性，这表明该系统中的修饰是DNA的甲基化。

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Bacillus subtilis-phage phi 1 overcomes host-controlled restriction by producing BamNx inhibitor protein.枯草芽孢杆菌噬菌体phi 1通过产生BamNx抑制蛋白克服宿主控制的限制。

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