Barghash Hind, Okedu Kenneth E, Al Balushi Aisha
Department of Engineering, German University of Technology, Al Athaibah, Oman.
Department of Electrical and Communication Engineering, National University of Science and Technology, Muscat, Oman.
Front Bioeng Biotechnol. 2021 Nov 17;9:644065. doi: 10.3389/fbioe.2021.644065. eCollection 2021.
Recently, it has become imperative to find new sustainable and renewable sources of energy, in order to avoid dependence on non-renewable traditional energy resources. This would help to overcome the depleting of natural resources for energy production. Hydrogen gas production using biological processes is one of the most attractive solutions in this regard, due to its high energy content and ecofriendly nature. Production of hydrogen using single photo-fermentation process and landfill leachate as substrate was carried out in this paper, by utilizing batch bio-reactor and anaerobic conditions. The pH value and temperature, play an essential role in a bio-hydrogen production process. Thus, in this study, the pH values considered were 6, 6.5, and 7.2, respectively, at a controlled temperature of 37 ± 1°C. This study investigated various schemes that have the possibility of producing hydrogen using; landfill leachate alone, with leachate and addition of inoculum such as sewage sludge, and with substrate such as sucrose and glucose. All experiments were conducted with and without mixing, for effective comparative study. Heat and pH pretreatment were applied in each experiment with the objectives of decreasing the activities of methane-producing bacteria and enhancing the activities of hydrogen-producing bacteria. The hydraulic retention time used in this study was 48 h, in order to obtain optimal performance of the schemes employed. Analysis of liquid leachate was performed for each experiment, and based on the obtained results, the maximum yield of hydrogen produced was 5,754 ml H/L, with a medium pH scale of 6.0, fermentation temperature of 37 ± 1°C and constant mixing speed of 100 rpm.
最近，寻找新的可持续和可再生能源已变得势在必行，以便避免依赖不可再生的传统能源资源。这将有助于克服能源生产中自然资源的枯竭。利用生物过程生产氢气是这方面最具吸引力的解决方案之一，因为其能量含量高且性质环保。本文利用分批生物反应器和厌氧条件，以单光发酵过程和垃圾渗滤液为底物进行了氢气生产。pH值和温度在生物制氢过程中起着至关重要的作用。因此，在本研究中，在37±1°C的控制温度下，分别考虑的pH值为6、6.5和7.2。本研究调查了各种利用以下方式生产氢气的方案：单独使用垃圾渗滤液、渗滤液与接种物（如污水污泥）混合以及与底物（如蔗糖和葡萄糖）混合。所有实验都在有混合和无混合的情况下进行，以进行有效的对比研究。在每个实验中都进行了加热和pH预处理，目的是降低产甲烷菌的活性并提高产氢菌的活性。本研究中使用的水力停留时间为48小时，以便获得所采用方案的最佳性能。对每个实验的液体渗滤液进行了分析，根据所得结果，在pH值为6.0、发酵温度为37±1°C和恒定搅拌速度为100 rpm的条件下，氢气的最大产量为5754毫升/升。
