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韩国野猪种群中猪繁殖与呼吸综合征的调查。

A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea.

作者信息

Choi Eun-Jin, Lee Chang-Hee, Hyun Bang-Hun, Kim Jae-Jo, Lim Seong-In, Song Jae-Young, Shin Yeun-Kyung

机构信息

Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-855, Korea.

出版信息

J Vet Sci. 2012 Dec;13(4):377-83. doi: 10.4142/jvs.2012.13.4.377.

Abstract

No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU, type 1) or North American (NA, type 2) viruses, respectively. Two amplicons (one from type 1 and one from type 2) were used to analyze the PRRSV open reading frame 7 (ORF7) sequence. The nucleotide sequences of type 1 PRRSV ORF7 had identities between 96.1% and 98.4% with PRRSVs from domestic pigs in Korea. The sequences of type 2 PRRSV ORF7 had identities of 100% with the PRRSV strain VR-2332, which was prototypic North American strain. These results show that PRRSVs are present in wild boars in Korea, and effective PRRSV surveillance of the wild boar population might therefore be useful for disease control.

摘要

目前尚无关于韩国野猪(Sus scrofa)感染猪繁殖与呼吸综合征病毒(PRRSV)的信息。在本研究中,对野猪的PRRS状况进行了调查。从韩国八个省份的267头野猪采集了血样。检测的样本中有4份(1.5%)PRRSV抗体呈阳性,8份(3.0%)抗原呈阳性。在病毒阳性样本中,分别有3份和5份样本被鉴定为含有欧洲(EU,1型)或北美(NA,2型)病毒。使用两个扩增子(一个来自1型,一个来自2型)分析PRRSV开放阅读框7(ORF7)序列。1型PRRSV ORF7的核苷酸序列与韩国家猪的PRRSV的同源性在96.1%至98.4%之间。2型PRRSV ORF7的序列与北美原型毒株PRRSV VR - 2332的同源性为100%。这些结果表明韩国野猪中存在PRRSV,因此对野猪群体进行有效的PRRSV监测可能有助于疾病控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b39/3539123/b7b19c9849d5/jvs-13-377-g001.jpg

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