Onizawa Katsuhiro, Muramatsu Takashi, Matsuki Miwako, Ohta Kazumasa, Matsuzaka Kenichi, Oda Yutaka, Shimono Masaki
Department of Pathology, Tokyo Dental College, Mihama-ku, Chiba, Japan.
Lasers Med Sci. 2009 Mar;24(2):155-61. doi: 10.1007/s10103-008-0541-7. Epub 2008 Jan 29.
We investigated cell response, including cell proliferation and expression of heat stress protein and bcl-2, to clarify the influence of low-level [gallium-aluminum-arsenide (Ga-Al-As) diode] laser irradiation on Par-C10 cells derived from the acinar cells of rat parotid glands. Furthermore, we also investigated amylase release and cell death from irradiation in acinar cells from rat parotid glands. The number of Par-C10 cells in the laser-irradiated groups was higher than that in the non-irradiated group at days 5 and 7, and the difference was statistically significant (P < 0.01). Greater expression of heat shock protein (HSP)25 and bcl-2 was seen on days 1 and 3 in the irradiated group. Assay of the released amylase showed no significant difference statistically between the irradiated group and the non-irradiated group. Trypan blue exclusion assay revealed that there was no difference in the ratio of dead to live cells between the irradiated and the non-irradiated groups. These results suggest that low-level laser irradiation promotes cell proliferation and expression of anti-apoptosis proteins in Par-C10 cells, but it does not significantly affect amylase secretion and does not induce rapid cell death in isolated acinar cells from rat parotid glands.
我们研究了细胞反应，包括细胞增殖以及热应激蛋白和bcl-2的表达，以阐明低强度[砷化镓铝（Ga-Al-As）二极管]激光照射对源自大鼠腮腺腺泡细胞的Par-C10细胞的影响。此外，我们还研究了大鼠腮腺腺泡细胞照射后的淀粉酶释放和细胞死亡情况。在第5天和第7天，激光照射组的Par-C10细胞数量高于未照射组，且差异具有统计学意义（P < 0.01）。照射组在第1天和第3天观察到热休克蛋白（HSP）25和bcl-2的表达更高。淀粉酶释放检测显示，照射组与未照射组之间在统计学上无显著差异。台盼蓝排斥试验表明，照射组和未照射组之间死细胞与活细胞的比例没有差异。这些结果表明，低强度激光照射可促进Par-C10细胞的增殖和抗凋亡蛋白的表达，但对淀粉酶分泌没有显著影响，也不会诱导大鼠腮腺分离腺泡细胞的快速细胞死亡。
