Simões Alyne, Ganzerla Emily, Yamaguti Paula Mochidome, de Paula Eduardo Carlos, Nicolau José
Oral Biology Research Center, Biomaterials and Oral Biochemistry Department, Faculty of Dentistry, University of São Paulo (USP), São Paulo, Brazil.
Lasers Med Sci. 2009 Jul;24(4):591-6. doi: 10.1007/s10103-008-0619-2. Epub 2008 Nov 4.
The aim of this study was to evaluate the effect of laser irradiation (LI) on enzymatic activities of amylase, catalase and peroxidase in the parotid glands (PG) of diabetic and non-diabetic rats. Ninety-six female rats were divided into eight groups: D0; D5; D10; D20 and C0; C5; C10; C20, respectively. Diabetes was induced by administration of streptozotocin and confirmed later by the glycemia results. Twenty-nine (29) days after the induction, the PGs of groups D5 and C5; D10 and C10; D20 and C20, were irradiated with 5 J/cm(2), 10 J/cm(2) and 20 J/cm(2) of laser diode (660 nm/100 mW) respectively. On the following day, the rats were euthanized and the enzymatic activity in the PGs was measured. Diabetic rats that had not been irradiated (group D0) showed higher catalase activity (P < 0.05) than those in group C0 (0.14 +/- 0.02 U/mg protein and 0.10 +/- 0.03 U/mg protein, respectively). However, laser irradiation of 5 J/cm(2) and 20 J/cm(2) decreased the catalase activity of the diabetic groups (D5 and D20) to non-diabetic values (P > 0.05). Based on the results of this study, LI decreased catalase activity in the PGs of diabetic rats.
本研究旨在评估激光照射（LI）对糖尿病和非糖尿病大鼠腮腺（PG）中淀粉酶、过氧化氢酶和过氧化物酶活性的影响。96只雌性大鼠被分为八组：分别为D0、D5、D10、D20和C0、C5、C10、C20。通过注射链脲佐菌素诱导糖尿病，随后通过血糖结果进行确认。诱导后29天，分别用5 J/cm²、10 J/cm²和20 J/cm²的激光二极管（660 nm/100 mW）对D5和C5、D10和C10、D20和C20组的腮腺进行照射。第二天，将大鼠安乐死并测量腮腺中的酶活性。未接受照射的糖尿病大鼠（D0组）的过氧化氢酶活性（P < 0.05）高于C0组（分别为0.14±0.02 U/mg蛋白和0.10±0.03 U/mg蛋白）。然而，5 J/cm²和20 J/cm²的激光照射使糖尿病组（D5和D20）的过氧化氢酶活性降至非糖尿病组水平（P > 0.05）。基于本研究结果，激光照射降低了糖尿病大鼠腮腺中的过氧化氢酶活性。
