In vitro analysis of peroxisomal protein import.
作者信息
Terlecky Stanley R
机构信息
Wayne State University School of Medicine, Detroit, Michigan, USA.
出版信息
Curr Protoc Cell Biol. 2002 May;Chapter 11:Unit 11.15. doi: 10.1002/0471143030.cb1115s14.
PMID:18228397
Abstract
This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.
摘要
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