Mahoney J J, Harvey J A, Wong R J, Van Kessel A L
Division of Pulmonary and Critical Care Medicine, Stanford University School of Medicine, CA 94305.
Clin Chem. 1991 Jul;37(7):1244-8.
Tonometered whole-blood and plasma specimens were tested in plastic and glass syringes to determine whether clinically significant changes in gas tensions occur during sample storage. When whole blood was tonometered with 60 and 100 mL/L (6% and 10%) oxygen and then stored for 30 min in iced plastic syringes, the pO2 of the samples remained stable (mean change = +0.4 and +0.8 mmHg, respectively). However, for 140 mL/L (14%) oxygen tonometry, the pO2 increased significantly (mean change = +8.4 mmHg; P less than 0.0001). When tonometered plasma was stored in iced plastic syringes, the pO2 increased progressively at all three concentrations, with the smallest change occurring at 140 mL/L (mean change = +12.6 mmHg) and the greatest at 60 mL/L oxygen (mean change = +20.9 mmHg). In contrast, when iced glass syringes were used for storing plasma or whole blood, no clinically significant changes in pO2 were found at any of the tonometered oxygen values for 60 min. When whole blood was stored in plastic syringes at ambient temperature for 30 min, again no clinically significant changes in pO2 were found at these tonometry conditions. Apparently, some blood gas samples stored in iced plastic syringes may yield clinically significant errors in oxygen tension.
将经眼压计测量的全血和血浆样本置于塑料和玻璃注射器中进行测试，以确定样本储存期间气体张力是否会发生具有临床意义的变化。当全血用60和100 mL/L（6%和10%）氧气进行眼压计测量，然后在冰浴的塑料注射器中储存30分钟时，样本的pO2保持稳定（平均变化分别为+0.4和+0.8 mmHg）。然而，对于140 mL/L（14%）氧气眼压计测量，pO2显著增加（平均变化 = +8.4 mmHg；P小于0.0001）。当经眼压计测量的血浆储存在冰浴的塑料注射器中时，在所有三种浓度下pO2均逐渐增加，在140 mL/L时变化最小（平均变化 = +12.6 mmHg），在60 mL/L氧气时变化最大（平均变化 = +20.9 mmHg）。相比之下，当使用冰浴的玻璃注射器储存血浆或全血时，在60分钟内，任何眼压计测量的氧气值下均未发现pO2有临床意义的变化。当全血在室温下储存在塑料注射器中30分钟时，在这些眼压计测量条件下同样未发现pO2有临床意义的变化。显然，一些储存在冰浴塑料注射器中的血气样本可能会在氧张力方面产生具有临床意义的误差。
