Bridge J, Leong S P, Burgess A C, Thompson F, Trent J M
Department of Pathology, University of Nebraska Medical Center, Omaha 68198.
Melanoma Res. 1991 Nov-Dec;1(4):289-96. doi: 10.1097/00008390-199111000-00008.
Multiple metastatic melanoma lesions from three patients were cytogenetically characterized in order to assess the degree of intra-patient karyotypic heterogeneity. A total of 20 specimens were analysed: 12 samples from patient No. 1, five samples from patient No. 2, and three samples from patient No. 3. Sufficient mitoses were obtained to perform detailed analysis in 19/20 specimens following short-term culture. The modal chromosome number of all three cases was near-diploid, with all samples demonstrating multiple structural abnormalities. Abnormalities shared by all three patients were alterations of chromosomes 1 and 8. Other structural abnormalities common to two of the three patients involved chromosomes 6, 7, 9 and 10. Minor intra-tumour karyotypic variation was detected in all three cases. However, the majority of clonal alterations were retained in all metastatic lesions, clearly indicating the karyotypically stable and clonal nature of this neoplasm.
对三名患者的多个转移性黑色素瘤病灶进行了细胞遗传学特征分析，以评估患者体内核型异质性的程度。共分析了20个样本：患者1的12个样本、患者2的5个样本和患者3的3个样本。在短期培养后，19/20个样本获得了足够的有丝分裂细胞以进行详细分析。所有三例的众数染色体数接近二倍体，所有样本均显示出多个结构异常。所有三名患者共有的异常是1号和8号染色体的改变。三名患者中有两名共有的其他结构异常涉及6、7、9和10号染色体。在所有三例中均检测到微小的肿瘤内核型变异。然而，大多数克隆性改变在所有转移性病灶中均得以保留，清楚地表明了该肿瘤在核型上的稳定性和克隆性。
