Lin Rong, Wang Wei-Rong, Liu Jun-Tian, Yang Guang-De, Han Chun-Jie
Department of Pharmacology, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, PR China.
J Ethnopharmacol. 2006 Nov 24;108(2):217-22. doi: 10.1016/j.jep.2006.05.004. Epub 2006 May 16.
Tanshinone IIA (Tan IIA) is isolated from Salvia miltiorrhiza, the root of which is widely used as a traditional Chinese medicine to treat atherosclerosis. The aim of the present study was to evaluate the putative protective effect of Tan IIA in a human umbilical vein endothelial cell line (ECV-304) injured by hydrogen peroxide in vitro and the mechanism of its protection. The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The endothelial cell apoptosis and expression of cluster of differentiation 40 (CD40) were detected by flow cytometric analysis. Preincubation with Tan IIA significantly increased the viability of ECV-304 cell injured by hydrogen peroxide, which was accompanied with the increased nitric oxide level and superoxide dismutase activity in a dose-dependent manner. Moreover, cell apoptosis and CD40 expression were decreased in a dose-dependent manner. In conclusion, our data suggests that Tan IIA protects ECV-304 cell damage induced by hydrogen peroxide through its anti-oxidant effect and CD40 anti-inflammatory approach.
丹参酮IIA（Tan IIA）从丹参中分离得到，丹参根作为一种传统中药被广泛用于治疗动脉粥样硬化。本研究的目的是评估Tan IIA在体外对过氧化氢损伤的人脐静脉内皮细胞系（ECV - 304）的假定保护作用及其保护机制。通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）法评估细胞活力百分比。通过流式细胞术分析检测内皮细胞凋亡和分化簇40（CD40）的表达。用Tan IIA预孵育显著提高了过氧化氢损伤的ECV - 304细胞的活力，同时一氧化氮水平和超氧化物歧化酶活性呈剂量依赖性增加。此外，细胞凋亡和CD40表达呈剂量依赖性降低。总之，我们的数据表明Tan IIA通过其抗氧化作用和CD40抗炎途径保护过氧化氢诱导的ECV - 304细胞损伤。
