胡黄连（玄参科）根茎对吲哚美辛诱导的胃溃疡的愈合潜力：一项机制探索。
Healing potential of Picrorhiza kurroa (Scrofulariaceae) rhizomes against indomethacin-induced gastric ulceration: a mechanistic exploration.
作者信息
Banerjee Debashish, Maity Biswanath, Nag Subrata K, Bandyopadhyay Sandip K, Chattopadhyay Subrata
机构信息
Bio-Organic Division, Bhabha Atomic Research Centre, Mumbai - 400 085, India.
出版信息
BMC Complement Altern Med. 2008 Jan 31;8:3. doi: 10.1186/1472-6882-8-3.
BACKGROUND
The present study was undertaken to evaluate the potential of the rhizomes of the Indian medicinal plant, Picrorhiza kurroa in healing indomethacin-induced acute stomach ulceration in mice and examine its capacity to modulate oxidative stress and the levels of prostaglandin (PGE2) and EGF during the process.
METHODS
Male swiss albino mice, ulcerated with indomethacin (18 mg/kg, p. o., single dose) were treated up to 7 days with different doses of the methanol extract of P. kurroa rhizomes (designated as PK). The healing capacity of the most effective dose of PK (20 mg/kg, p. o. x 3 d) was compared with that of omeprazole (Omez) (3 mg/kg, p. o. x 3 d). The effects of the drug-treatment for one and three days on the biochemical parameters were assessed by comparing the results with that of untreated mice of the 1st and 3rd day of ulceration. The stomach tissues of the mice were used for the biochemical analysis.
RESULTS
The macroscopic indices revealed maximum ulceration on the 3rd day after indomethacin administration, which was effectively healed by PK. Under the optimized treatment regime, PK and Omez reduced the ulcer indices by 45.1% (P < 0.01), and 76.3% respectively (P < 0.001), compared to the untreated ulcerated mice. Compared to the ulcerated untreated mice, those treated with PK for 3 days showed decreased the levels of thiobarbituric acid reactive substances (TBARS) (32.7%, P < 0.05) and protein carbonyl (37.7%, P < 0.001), and increased mucin (42.2%, P < 0.01), mucosal PGE2 (21.4%, P < 0.05), and expressions of COX-1 and 2 (26.9% and 18.5%, P < 0.05), EGF (149.0%, P < 0.001) and VEGF (56.9%, P < 0.01). Omez reduced the TBARS (29.4%, P < 0.05), and protein carbonyl (38.9%, P < 0.001), and increased mucin (38.3%, P < 0.01), without altering the other parameters significantly.
CONCLUSION
PK (20 mg/kg, p. o. x 3 days) could effectively heal indomethacin-induced stomach ulceration in mice by reducing oxidative stress, and promoting mucin secretion, prostaglandin synthesis and augmenting expressions of cyclooxygenase enzymes and growth factors.
背景
本研究旨在评估印度药用植物胡黄连根茎在治疗吲哚美辛诱导的小鼠急性胃溃疡方面的潜力，并研究其在该过程中调节氧化应激以及前列腺素（PGE2）和表皮生长因子（EGF）水平的能力。
方法
用吲哚美辛（18mg/kg，口服，单剂量）诱导雄性瑞士白化小鼠产生溃疡，然后用不同剂量的胡黄连根茎甲醇提取物（称为PK）治疗7天。将最有效剂量的PK（20mg/kg，口服×3天）的愈合能力与奥美拉唑（Omez）（3mg/kg，口服×3天）进行比较。通过将药物治疗1天和3天的生化参数结果与溃疡第1天和第3天未治疗小鼠的结果进行比较，来评估药物治疗的效果。小鼠的胃组织用于生化分析。
结果
宏观指标显示，吲哚美辛给药后第3天溃疡最严重，而PK可有效治愈溃疡。在优化的治疗方案下，与未治疗的溃疡小鼠相比，PK和Omez分别使溃疡指数降低了45.1%（P<0.01）和76.3%（P<0.001）。与未治疗的溃疡小鼠相比，用PK治疗3天的小鼠硫代巴比妥酸反应性物质（TBARS）水平降低（32.7%，P<0.05），蛋白质羰基水平降低（37.7%，P<0.001），粘蛋白增加（42.2%，P<0.01），粘膜PGE2增加（21.4%，P<0.05），环氧化酶-1和-2的表达增加（26.9%和18.5%，P<0.05），EGF增加（149.0%，P<0.001），血管内皮生长因子增加（56.9%，P<0.01）。Omez降低了TBARS（29.4%，P<0.05）和蛋白质羰基（38.9%，P<0.001），增加了粘蛋白（38.3%，P<0.01），但对其他参数没有显著影响。
结论
PK（20mg/kg，口服×3天）可通过降低氧化应激、促进粘蛋白分泌、前列腺素合成以及增加环氧化酶和生长因子的表达，有效治愈吲哚美辛诱导的小鼠胃溃疡。
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