蒌叶酚、烯丙基邻苯二酚对消炎痛诱导的胃溃疡的愈合特性及作用机制。
Healing property of the Piper betel phenol, allylpyrocatechol against indomethacin-induced stomach ulceration and mechanism of action.
作者信息
Bhattacharya S, Banerjee D, Bauri A-K, Chattopadhyay S, Bandyopadhyay S-K
机构信息
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences and IPGMERR, 244B, Acharya Jagadish Chandra Bose Road, Kolkata 700020, India.
出版信息
World J Gastroenterol. 2007 Jul 21;13(27):3705-13. doi: 10.3748/wjg.v13.i27.3705.
AIM
To evaluate the protective activity of allylpyrocatechol (APC), the major antioxidant constituent of Piper betel, against the indomethacin-induced stomach ulceration in the rat model and correlates with its antioxidative and mucin protecting properties.
METHODS
Male Sprague-Dawley rats were divided into five groups. Normal control rats (group I) were given the vehicle oral dose of gum acacia in distilled water (1 mL per rat); ulcerated control and treated rats (groups II-V) were given a single dose of indomethacin (30 mg/kg body wt.); group II rats were sacrificed 4 h after indomethacin administration; groups III-V rats were given the vehicle (1 mL per rat) or APC (2 mg/kg body wt.) or misoprostol (1.43 mug/kg body wt.) once daily by oral intubation for 7 d starting from 4 h after the indomethacin administration. After 7 d, the stomach tissues were excised for histological examination and biochemical analysis.
RESULTS
Treatment with APC (2 mg/kg body wt per day) and misoprostol (1.43 mug/kg body wt per day) for 7 d could effectively heal the stomach ulceration as revealed from the ulcer index and histopathological studies. Compared to the zero day ulcerated group, treatment with APC and misoprostol reduced the ulcer index by 93.4% and 85.4% respectively (P < 0.05). Both APC and misoprostol accelerated ulcer healing observed in natural recovery (P < 0.05), their respective healing capacities not being significantly different. The healing capacities of APC and misoprostol could be attributed to their antioxidant activity as well as the ability to enhance the mucin content of the gastric tissues. Compared to the ulcerated untreated rats, those treated with APC and misoprostol showed near normal MDA levels, while the protein levels were 86% and 78% of the normal value respectively (P < 0.05). Likewise, both APC and misoprostol increased the SOD, catalase, and mucin levels significantly (P < 0.05), the effect of APC being better.
CONCLUSION
APC can protect indomethacin-induced gastric ulceration due to its antioxidative and mucin protecting properties.
目的
评估蒌叶主要抗氧化成分烯丙基邻苯二酚（APC）对吲哚美辛诱导的大鼠胃溃疡的保护作用，并探讨其与抗氧化及保护黏液特性的相关性。
方法
将雄性Sprague-Dawley大鼠分为五组。正常对照组（I组）给予蒸馏水配制的阿拉伯胶口服剂量（每只大鼠1 mL）；溃疡对照组和治疗组（II-V组）给予单剂量吲哚美辛（30 mg/kg体重）；II组大鼠在给予吲哚美辛4小时后处死；III-V组大鼠从给予吲哚美辛4小时后开始，每天经口插管给予载体（每只大鼠1 mL）或APC（2 mg/kg体重）或米索前列醇（1.43 μg/kg体重），持续7天。7天后，切除胃组织进行组织学检查和生化分析。
结果
从溃疡指数和组织病理学研究结果显示，每天给予APC（2 mg/kg体重）和米索前列醇（1.43 μg/kg体重）治疗7天可有效治愈胃溃疡。与第0天溃疡组相比，APC和米索前列醇治疗分别使溃疡指数降低了93.4%和85.4%（P < 0.05）。APC和米索前列醇均加速了自然恢复过程中的溃疡愈合（P < 0.05），它们各自的愈合能力无显著差异。APC和米索前列醇的愈合能力可归因于它们的抗氧化活性以及提高胃组织黏液含量的能力。与未治疗的溃疡大鼠相比，接受APC和米索前列醇治疗的大鼠丙二醛（MDA）水平接近正常，而蛋白质水平分别为正常值的86%和78%（P < 0.05）。同样地，APC和米索前列醇均显著提高了超氧化物歧化酶（SOD）、过氧化氢酶和黏液水平（P < 0.05），APC的效果更佳。
结论
APC因其抗氧化和保护黏液的特性，可保护吲哚美辛诱导的胃溃疡。
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