Scher C, Haudenschild C, Klagsbrun M
Cell. 1976 Jul;8(3):373-82. doi: 10.1016/0092-8674(76)90149-5.
The chick chorioallantoic membrane (CAM) was used as an assay system to investigate the the invasive properties of viral transformed NIH/3Y3 cells. Scanning electron microscopy demonstrated that single Kirsten sarcoma virus (KiSV)-transformed cells passed between the epithelial cells of the CAM ectoderm within 6 hr of application, while viable NIH/3T3 cells did not penetrate the ectoderm within 24 hr. The transformed cells entered the mesoderm of the CAM and formed tumors of proliferating cells. The application of 5 X 10(5) KiSV-transformed cells resulted in the formation of donor cells resulted in the formation of the donor cell tumors within 5 days in 43% of the membranes. No tumors were formed when as many as 5 X 10(6) NIH/3T3 cells were applied to the membrane. NIH/3T3 cells transformed by the Abelson leukemia virus or the Moloney sarcoma virus also ivaded the CAM and formed tumors of proliferating cells within the mesoderm, while cells infected with the Moloney leukemia virus did not. NIH/3T3 cells inoculated onto the CAM 8 days after infection and transformation with KiSV formed tumors with a frequency similar to that of KiSV transformed cells that have been passaged in culture for many generations. Cells that formed invasive tumors within the mesoderm also attracted loops of host blood vessels.
鸡胚绒毛尿囊膜（CAM）被用作一种检测系统，以研究病毒转化的NIH/3Y3细胞的侵袭特性。扫描电子显微镜显示，单个 Kirsten 肉瘤病毒（KiSV）转化的细胞在接种后6小时内穿过CAM外胚层的上皮细胞，而活的 NIH/3T3细胞在24小时内未穿透外胚层。转化细胞进入CAM的中胚层并形成增殖细胞肿瘤。接种5×10⁵个KiSV转化细胞，43%的膜在5天内形成供体细胞肿瘤。当向膜上接种多达5×10⁶个NIH/3T3细胞时，未形成肿瘤。由Abelson白血病病毒或Moloney肉瘤病毒转化的NIH/3T3细胞也侵袭CAM并在中胚层内形成增殖细胞肿瘤，而感染Moloney白血病病毒的细胞则不会。在感染并被KiSV转化8天后接种到CAM上的NIH/3T3细胞形成肿瘤的频率与在培养中传代许多代的KiSV转化细胞相似。在中胚层内形成侵袭性肿瘤的细胞也吸引宿主血管环。
