Sweeny P R, Bather R
J Cell Biol. 1968 Feb;36(2):299-311. doi: 10.1083/jcb.36.2.299.
Infection of the chick chorioallantoic membrane (CAM) with Rous sarcoma virus (RSV) has been thought by earlier workers (12, 20) to result in the transformation of the ectoderm and then the mesoderm of that organ. In the present study, CAM were infected with 10(4) PFU (pock-forming units) of RSV (Bryan high titre strain) and collected for electron microscopy at 2, 4, and 6 days postinfection. Observations of the fine structural changes in the CAM after RSV infection support a singular role of the mesenchyme in the initiation of the tumors. The ectodermal hyperplasia often associated with RSV tumors of the CAM appears to be a secondary response to the alteration of the underlying mesenchyme. These findings are discussed in detail, and an alternate course of RSV transformation of the CAM by way of the vascular bed is suggested.
早期研究人员（12, 20）认为，用劳斯肉瘤病毒（RSV）感染鸡胚绒毛尿囊膜（CAM）会导致该器官的外胚层，进而中胚层发生转化。在本研究中，用10⁴ 蚀斑形成单位（PFU）的RSV（布莱恩高滴度株）感染CAM，并在感染后第2、4和6天收集用于电子显微镜观察。对RSV感染后CAM的细微结构变化的观察支持间充质在肿瘤发生起始过程中起单一作用。CAM的RSV肿瘤常伴有的外胚层增生似乎是对其下方间充质改变的继发反应。本文详细讨论了这些发现，并提出了RSV通过血管床使CAM发生转化的另一种途径。
