Jung K, Priem F, Klotzek S, Becker S, Henke W
Department of Experimental Organ Transplantation, University Hospital Charité, Humboldt University Berlin, FRG.
Enzyme. 1991;45(4):215-21. doi: 10.1159/000468892.
N-Acetyl-beta-D-glucosaminidase (NAG) activities in the urine of men and rats were measured with methods recommended as procedures without pretreatment of the urine sample. Four different derivatives of NAG were compared for determination: 4-nitrophenyl; 3,3-dichlorophenylsulfonphthaleinyl; 3-cresolsulfonphthaleinyl, and 2-methoxy-4-(2-nitro-vinyl)phenyl. The conventional test using the 4-nitrophenyl derivative showed the highest activities and correlated very well with the other tests. There are method-dependent differences between NAG activities measured in men and rats due to the different Km values and inhibitory effects by urea.
采用推荐的无需对尿液样本进行预处理的方法，测定了男性和大鼠尿液中的N-乙酰-β-D-氨基葡萄糖苷酶（NAG）活性。比较了用于测定的四种不同的NAG衍生物：4-硝基苯基；3,3-二氯苯基磺酞基；3-甲酚磺酞基和2-甲氧基-4-（2-硝基乙烯基）苯基。使用4-硝基苯基衍生物的传统测试显示出最高活性，并且与其他测试相关性非常好。由于不同的米氏常数（Km）值和尿素的抑制作用，男性和大鼠中测得的NAG活性存在方法依赖性差异。
