Nias A H, Fox B W
Chem Biol Interact. 1976 Jul;14(1-2):93-9. doi: 10.1016/0009-2797(76)90027-2.
HeLa and Chinese hamster (ovary) cells were exposed in vitro to methylene dimethanesulphonate (MDMS) and their survival of colony-forming ability was assayed in monolayer culture. Asynchronous cultures were exposed to the drug for the whole culture period but cell survival was complicated by the toxicity of formaldehyde which is a final breakdown product of the drug. A short treatment schedule of 15 min within the hydrolytic half life of the agent was therefore employed and the response of synchronous cultures of HeLa cells was then assayed throughout the course of the cells cycle. Cells were most sensitive at the beginning of the DNA synthetic phase (early-S) and most resistant at the end (late-S).
将HeLa细胞和中国仓鼠（卵巢）细胞在体外暴露于甲撑二甲磺酸盐（MDMS），并在单层培养中测定其集落形成能力的存活率。将异步培养物在整个培养期内暴露于该药物，但由于甲醛是该药物的最终分解产物，其毒性使细胞存活情况变得复杂。因此，采用在该试剂水解半衰期内15分钟的短处理方案，然后在整个细胞周期过程中测定HeLa细胞同步培养物的反应。细胞在DNA合成期开始时（早S期）最敏感，在结束时（晚S期）最耐药。
