Crathorn A R, Shackleton J
Chem Biol Interact. 1976 Oct 2;15(2):117-30. doi: 10.1016/0009-2797(76)90157-5.
A method for producing a viable non-dividing population of Chinese hamster V79 cells in suspension is described and the characteristics of the population outlined. The stationary population is more sensitive to methylating agents than a similar but exponentially growing population, the increased sensitivity arising from the loss of the shoulder from the survival curve. The extent of reaction of the agent with cellular macromolecules is similar in both cases. The repair capabilities of the two populations was examined. Non-semiconservative DNA repair synthesis occurs whether the cells are in a growth or no-growth condition when insulted. Repair of single-strand breaks, which arise following methylation, also proceeds up to the size of the replicon. The relationship of this stationary population to other no-growth conditions and its utility as a model for carcinogenesis studies is discussed.
本文描述了一种在悬浮状态下产生可行的非分裂中国仓鼠V79细胞群体的方法，并概述了该群体的特征。静止期群体比类似的指数生长期群体对甲基化剂更敏感，这种敏感性增加是由于存活曲线中肩部的消失所致。在两种情况下，该试剂与细胞大分子的反应程度相似。研究了这两个群体的修复能力。当受到损伤时，无论细胞处于生长状态还是非生长状态，都会发生非半保留性DNA修复合成。甲基化后产生的单链断裂的修复也会进行到复制子的大小。讨论了这个静止期群体与其他非生长条件的关系及其作为致癌研究模型的实用性。
