Zhou M H, Han G Z, Chu Y H
Department of Pharmacology, Shanghai Medical University.
Yao Xue Xue Bao. 1991;26(11):801-4.
Placental villus tissue obtained from pregnant women of 6 to 7 weeks was cultured in vitro for four days, with or without addition of tamoxifen (Tam), an antiestrogenic drug. The levels of both progesterone (P) and human chorionic gonadotropin (hCG) in culture medium collected every day were measured by using radio-immuno assay, so as to observe the effects of Tam on the secretion of P and hCG by villus tissue as well as the time course of these effects. The experimental results showed that the levels of both P and hCG in the culture medium collected on the 3rd and 4th days in the Tam-treated group were significantly lower than those in the control group, indicating that Tam could have a direct inhibitory effect on the secretory function of the placental villus tissue during early pregnancy. The slow onset of the inhibitory effect was in correspondence with the clinical observation that the early pregnancy terminating effect of PGS would be potentiated after 4-5 days of Tam medication. In addition, this inhibitory effect of tamoxifen on the placental secretory function was suggested to be associated with a decrease in the responsiveness of placental tissue to GnRH.
从怀孕6至7周的孕妇获取胎盘绒毛组织，在体外培养4天，添加或不添加抗雌激素药物他莫昔芬（Tam）。每天收集的培养基中的孕酮（P）和人绒毛膜促性腺激素（hCG）水平采用放射免疫分析法进行测定，以观察他莫昔芬对绒毛组织分泌P和hCG的影响以及这些影响的时间进程。实验结果表明，他莫昔芬处理组第3天和第4天收集的培养基中P和hCG水平均显著低于对照组，表明他莫昔芬对妊娠早期胎盘绒毛组织的分泌功能可能有直接抑制作用。抑制作用起效缓慢，这与临床观察结果相符，即服用他莫昔芬4 - 5天后，PGS的早期妊娠终止作用会增强。此外，他莫昔芬对胎盘分泌功能的这种抑制作用被认为与胎盘组织对促性腺激素释放激素（GnRH）反应性降低有关。
