Zhu B T, Chu Y H
Department of Pharmacology, Faculty of Basic Medical Science, Shanghai Medical University.
Yao Xue Xue Bao. 1990;25(6):469-72.
In the present study, the method of collagenase-pepsin prepared cell suspension of human placental villi from early (10-12 weeks) and term (38-40 weeks) gestation cultured in vitro combined with progesterone radioimmunoassay was employed. The results indicated that: 1. In the placenta villi cells from early and term gestation in vitro, both exogenous and endogenous hCG showed a stimulatory effect on progesterone secretion, and the effect was approximately proportional to the concentration of the exogenous hCG added. 2. In the placenta villi cells from term gestation in vitro, after addition of sufficient rabbit antihCG IgG to the culture medium, LHRH-A exhibited no obvious influence on this hCG-independent progesterone secretion. However, in the absence of rabbit anti-hCG IgG in the culture medium, LHRH-A showed a stimulatory action on progesterone secretion probably via its stimulatory effect on hCG secretion which could subsequently increase the progesterone secretion. 3. In the placenta villi cells from early gestation, LHRH-A exhibited an inhibitory effect on both hCG-independent and hCG-dependent progesterone secretion.
在本研究中，采用胶原酶-胃蛋白酶法制备人早孕期（10 - 12周）和足月孕期（38 - 40周）胎盘绒毛的细胞悬液，进行体外培养，并结合孕酮放射免疫测定法。结果表明：1. 早孕期和足月孕期胎盘绒毛细胞在体外，外源性和内源性hCG均对孕酮分泌有刺激作用，且该作用与添加的外源性hCG浓度大致成正比。2. 在足月孕期胎盘绒毛细胞体外培养中，向培养基中加入足量兔抗hCG IgG后，LHRH - A对这种不依赖hCG的孕酮分泌无明显影响。然而，在培养基中不存在兔抗hCG IgG时，LHRH - A可能通过刺激hCG分泌，进而增加孕酮分泌，对孕酮分泌有刺激作用。3. 在早孕期胎盘绒毛细胞中，LHRH - A对不依赖hCG和依赖hCG的孕酮分泌均有抑制作用。
