褪黑素可减少三硝基苯磺酸诱导的大鼠结肠炎中的细菌移位和细胞凋亡。
Melatonin reduces bacterial translocation and apoptosis in trinitrobenzene sulphonic acid-induced colitis of rats.
作者信息
Akcan Alper, Kucuk Can, Sozuer Erdogan, Esel Duygu, Akyildiz Hizir, Akgun Hulya, Muhtaroglu Sabahattin, Aritas Yucel
机构信息
Department of General Surgery, Erciyes University School of Medicine, Kayseri 38039, Turkey.
出版信息
World J Gastroenterol. 2008 Feb 14;14(6):918-24. doi: 10.3748/wjg.14.918.
AIM
To investigate the effects of exogenous melatonin on bacterial translocation and apoptosis in a rat ulcerative colitis model.
METHODS
Rats were randomly assigned to three groups: group I: control, group II: experimental colitis, group III: colitis plus melatonin treatment. On d 11 after colitis, plasma tumor necrosis factor-alpha, portal blood endotoxin levels, colon tissue myeloperoxidase and caspase-3 activity were measured. Bacterial translocation was quantified by blood, lymph node, liver and spleen culture.
RESULTS
We observed a significantly reduced incidence of bacterial translocation to the liver, spleen, mesenteric lymph nodes, portal and systemic blood in animals treated with melatonin. Treatment with melatonin significantly decreased the caspase-3 activity in colonic tissues compared to that in trinitrobenzene sulphonic acid- treated rats (16.11 +/- 2.46 vs 32.97 +/- 3.91, P < 0.01).
CONCLUSION
Melatonin has a protective effect on bacterial translocation and apoptosis.
目的
研究外源性褪黑素对大鼠溃疡性结肠炎模型中细菌移位和细胞凋亡的影响。
方法
将大鼠随机分为三组：第一组：对照组；第二组：实验性结肠炎组；第三组：结肠炎加褪黑素治疗组。在结肠炎发病后第11天，检测血浆肿瘤坏死因子-α、门静脉血内毒素水平、结肠组织髓过氧化物酶和半胱天冬酶-3活性。通过血液、淋巴结、肝脏和脾脏培养对细菌移位进行定量分析。
结果
我们观察到，接受褪黑素治疗的动物中，细菌移位至肝脏、脾脏、肠系膜淋巴结、门静脉和全身血液的发生率显著降低。与三硝基苯磺酸处理的大鼠相比，褪黑素治疗显著降低了结肠组织中的半胱天冬酶-3活性（16.11±2.46对32.97±3.91，P<0.01）。
结论
褪黑素对细菌移位和细胞凋亡具有保护作用。
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