Konnai Satoru, Mingala Claro N, Sato Misako, Abes Nancy S, Venturina Fe A, Gutierrez Charito A, Sano Takafumi, Omata Yoshitaka, Cruz Libertado C, Onuma Misao, Ohashi Kazuhiko
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Acta Trop. 2008 Mar;105(3):269-73. doi: 10.1016/j.actatropica.2007.12.004. Epub 2007 Dec 23.
In the Philippines, insufficient consideration has been given to the implementation of systematic control measures against major abortifacient infectious agents in livestock. To elucidate the epidemiology of abortifacient infectious agents in livestock, the prevalence of four abortifacient agents was assessed. Initially, a total of 96 cattle including 17 cows with history of abortion were examined in a herd in Luzon at the request of the farm owner. Six (35.3%) of the 17 aborting cows were found to be serologically positive for Neospora caninum (N. caninum), whereas the seroprevalence in non-aborting cows was 15.9% (10/63). Four of the 6 serologically positive aborting cows were also RT-PCR-positive for bovine viral diarrhea virus (BVDV). Two (12.5%) of the 16 bulls examined were also found to be infected with BVDV, suggesting a putative risk factor of transmission via semen. Based on sequence analysis, the isolates detected belong to BVDV type 1b group. Furthermore, an epidemiological survey of abortifacient infectious agents was conducted with various species of livestock from herds located in Luzon. Out of the 105 water buffalo samples collected, 4 (3.8%) were indicated positive to N. caninum, 2 (1.9%) to Toxoplasma gondii (T. gondii) and 2 (1.9%) to Trypanosoma evansi (T. evansi). The overall seroprevalence of N. caninum in goat and sheep were 23.6% (21/89) and 26.3% (10/38), respectively. BVDV was not detected in these herds. The findings of this exploratory study indicate a relationship between infection and bovine abortion and that a lager study is required to statistically confirm this relationship.
在菲律宾，对家畜主要流产性感染因子实施系统控制措施的考虑尚不充分。为阐明家畜流产性感染因子的流行病学情况，对四种流产性因子的流行情况进行了评估。最初，应农场主的要求，在吕宋岛的一个牛群中对总共96头牛进行了检查，其中包括17头有流产史的母牛。17头流产母牛中有6头（35.3%）经血清学检测显示犬新孢子虫（N. caninum）呈阳性，而未流产母牛的血清阳性率为15.9%（10/63）。6头血清学阳性的流产母牛中有4头经逆转录聚合酶链反应（RT-PCR）检测显示牛病毒性腹泻病毒（BVDV）呈阳性。在检查的16头公牛中，有2头（12.5%）也被发现感染了BVDV，这表明存在通过精液传播的潜在风险因素。基于序列分析，检测到的分离株属于BVDV 1b型组。此外，还对吕宋岛各牛群中的不同家畜品种进行了流产性感染因子的流行病学调查。在采集的105份水牛样本中，4份（3.8%）对犬新孢子虫呈阳性，2份（1.9%）对刚地弓形虫（T. gondii）呈阳性，2份（1.9%）对伊氏锥虫（T. evansi）呈阳性。山羊和绵羊中犬新孢子虫的总体血清阳性率分别为23.6%（21/89）和26.3%（10/38）。在这些牛群中未检测到BVDV。这项探索性研究的结果表明感染与牛流产之间存在关联，需要进行更大规模的研究以从统计学上证实这种关联。
