1986年至1995年瑞士牛流产的病因——一项侧重于通过聚合酶链反应检测犬新孢子虫和弓形虫的回顾性研究
Aetiology of bovine abortion in Switzerland from 1986 to 1995--a retrospective study with emphasis on detection of Neospora caninum and Toxoplasma gondii by PCR.
作者信息
Reitt K, Hilbe M, Voegtlin A, Corboz L, Haessig M, Pospischil A
机构信息
Institute of Veterinary Pathology, Winterthurerstr. 268, Zuerich, Switzerland.
出版信息
J Vet Med A Physiol Pathol Clin Med. 2007 Feb;54(1):15-22. doi: 10.1111/j.1439-0442.2007.00913.x.
In a retrospective study, covering the period from 1986 to 1995, tissues of aborted fetuses were re-examined. A total of 347 cases were tested immunohistochemically, among them samples of 223 brains were examined for Neospora caninum, Toxoplasma gondii and Bovine Virus Diarrhoea Virus (BVDV), and 249 placentae for Chlamydiaceae. Two real-time PCR assays, one for N. caninum, and one for T. gondii, were developed. These potential abortion-inducing agents were detected - and confirmed by PCR, except for BVDV - in 16.1% (N. caninum), 0% (T. gondii), 9.9% (BVDV) and 0.8% (Chlamydiales) of the cases examined. Immunohistochemistry proved to be inadequate for the detection of the protozoal epitopes, whereas it was confirmed as a very useful tool for the detection of BVDV. In abortion material, PCR is considered to be more suitable for the detection of protozoa and Chlamydophila abortus, an adequate sampling presupposed.
在一项涵盖1986年至1995年期间的回顾性研究中，对流产胎儿的组织进行了重新检查。总共对347例样本进行了免疫组织化学检测，其中对223例脑样本检测了犬新孢子虫、弓形虫和牛病毒性腹泻病毒（BVDV），对249例胎盘检测了衣原体。开发了两种实时PCR检测方法，一种用于检测犬新孢子虫，另一种用于检测弓形虫。在所检测的病例中，除BVDV外，这些潜在的致流产病原体通过PCR检测到并得到确认，其中犬新孢子虫为16.1%，弓形虫为0%，BVDV为9.9%，衣原体为0.8%。免疫组织化学被证明不足以检测原生动物表位，而被确认为检测BVDV的非常有用的工具。在流产材料中，假定采样充分，PCR被认为更适合检测原生动物和流产嗜衣原体。
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