Sádaba Luis M, Fernández-Robredo Patricia, Rodríguez José Antonio, García-Layana Alfredo
Department of Ophthalmology, School of Medicine, University of Navarra, Pamplona, Spain.
Exp Eye Res. 2008 Mar;86(3):470-9. doi: 10.1016/j.exer.2007.11.020. Epub 2007 Dec 15.
The aim of the current study was to investigate the biochemical changes in the plasma and retina of apolipoprotein E deficient (apoE-/-) mice supplemented with various antioxidants. Ten wild type (WT-Con, C57BL/6) and 10 apoE-/- (AE-Con) mice received drinking water. Another 40 apoE-/- animals were divided into four groups of 10 mice each and received either chromocarbe diethylamine (AE-CD, 50mg/kg), cyaninosides chloride (AE-CC, 50mg/kg), multivitamin complex (AE-MC, 50mg/kg), or vitamins C and E (AE-CE, 100mg/kg and 200IU/kg). Cholesterol, triglycerides, and lipid peroxidation (thiobarbituric acid reactive substances [TBARS]) were measured in plasma, and TBARS and nitric oxide metabolites (NOx) concentration were determined in retinal homogenates. Transmission electron microscopy was performed to examine the retinal ultrastructure. AE-Con mice had significantly (P<0.05) increased oxidative stress in the plasma and retina with augmented production of retinal NOx compared with WT-Con mice. Retinal TBARS decreased in the AE-MC and AE-CE animals compared with the AE-Con group (P<0.05 and P<0.01, respectively). Only AE-CE treatment significantly (P<0.01) lowered retinal NOx. Morphologic retinal changes in the AE-Con group decreased in the AE-CE and AE-MC groups. There were no significant changes in the biochemical and structural parameters in the AE-CD and AE-CC groups. AE-Con mice had increased systemic and retinal oxidative stress compared with WT-Con animals. Vitamins C and E and the multivitamin-mineral complex reduced oxidative stress and ultrastructural retinal changes in this murine model of hypercholesterolemia.
本研究的目的是调查补充各种抗氧化剂的载脂蛋白E缺陷（apoE-/-）小鼠血浆和视网膜中的生化变化。10只野生型（WT-Con，C57BL/6）和10只apoE-/-（AE-Con）小鼠饮用普通水。另外40只apoE-/-动物分为四组，每组10只，分别给予二乙胺色卡贝（AE-CD，50mg/kg）、氯化花青素（AE-CC，50mg/kg）、多种维生素复合物（AE-MC，50mg/kg）或维生素C和E（AE-CE，100mg/kg和200IU/kg）。检测血浆中的胆固醇、甘油三酯和脂质过氧化（硫代巴比妥酸反应性物质[TBARS]），并测定视网膜匀浆中的TBARS和一氧化氮代谢产物（NOx）浓度。进行透射电子显微镜检查以观察视网膜超微结构。与WT-Con小鼠相比，AE-Con小鼠血浆和视网膜中的氧化应激显著增加（P<0.05），视网膜NOx生成增多。与AE-Con组相比，AE-MC和AE-CE组动物的视网膜TBARS降低（分别为P<0.05和P<0.01）。只有AE-CE治疗显著降低了视网膜NOx（P<0.01）。AE-Con组的视网膜形态学变化在AE-CE和AE-MC组中有所减轻。AE-CD和AE-CC组的生化和结构参数无显著变化。与WT-Con动物相比，AE-Con小鼠的全身和视网膜氧化应激增加。在这种高胆固醇血症小鼠模型中，维生素C和E以及多种维生素-矿物质复合物可降低氧化应激和视网膜超微结构变化。
