Fleury C, Marin F, Marie B, Luquet G, Thomas J, Josse C, Serpentini A, Lebel J M
Université de Caen Basse Normandie, Esplanade de la Paix, 14032 CAEN Cedex, France.
Tissue Cell. 2008 Jun;40(3):207-18. doi: 10.1016/j.tice.2007.12.002. Epub 2008 Feb 19.
In the present paper, juvenile and adult shells of the green ormer Haliotis tuberculata ('Oreille de Saint-Pierre') were perforated in a zone close to the shell edge and the shell repair process was followed at two levels: (1) by observing the histology of the calcifying mantle in the repair zone and (2) by analyzing with SEM the microstructure of the shell repair zone. Histological data clearly show the presence of calcium carbonate granules into the connective tissues, but not in the epithelial cells. This suggests that calcium carbonate granules are synthesized by sub-epithelial cells and actively transported through the epithelium to the repair zone, via a process which may be similar to that described by Mount et al. [Mount, A.S., Wheeler, A.P., Paradkar, R.P., Snider, D., 2004. Hemocyte-mediated shell mineralization in the eastern oyster. Science 304, 297-300]. Furthermore, SEM observations show that the repair zone exhibits different stratified microstructures (spherulitic, thin prismatic, blocklike, sub-nacreous, nacreous, foliated-like), some of which are not continuous (i.e. lenticular) along the repair zone. This suggests a complex secreting regime of the calcifying mantle and an elaborate geometry of the epithelium involved in shell repair.
在本论文中，对绿盘鲍（Haliotis tuberculata，即“圣皮埃尔之耳”）的幼体和成体贝壳在靠近壳边缘的区域进行穿孔，并从两个层面跟踪贝壳修复过程：（1）通过观察修复区域钙化外套膜的组织学，以及（2）通过扫描电子显微镜（SEM）分析贝壳修复区域的微观结构。组织学数据清楚地显示碳酸钙颗粒存在于结缔组织中，而非上皮细胞中。这表明碳酸钙颗粒是由上皮下细胞合成，并通过一个可能类似于Mount等人[Mount, A.S., Wheeler, A.P., Paradkar, R.P., Snider, D., 2004. 东部牡蛎中血细胞介导的贝壳矿化。《科学》304, 297 - 300]所描述的过程，通过上皮细胞主动运输至修复区域。此外，扫描电子显微镜观察表明，修复区域呈现出不同的分层微观结构（球粒状、薄柱状、块状、亚珍珠质、珍珠质、叶状），其中一些在修复区域沿不连续（即透镜状）分布。这表明钙化外套膜存在复杂的分泌机制，以及参与贝壳修复的上皮细胞具有精细的几何结构。
