Darias M J, Zambonino-Infante J L, Hugot K, Cahu C L, Mazurais D
Ifremer, Nutrition Aquaculture and Genomics Research Unit, UMR 1067, Ifremer, Technopole Brest-Iroise, BP 70, 29280 Plouzané, France.
Mar Biotechnol (NY). 2008 Jul-Aug;10(4):416-28. doi: 10.1007/s10126-007-9078-1. Epub 2008 Feb 2.
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were generally up-expressed early, between 7 and 23 dph, and genes up-expressed between 25 and 43 dph. Interestingly, among the genes regulated during the larval period, those related to organogenesis, energy pathways, biosynthesis, and digestion were over-represented compared with total set of analyzed genes. We discuss the quantitative regulation of whole-body contents of these specific transcripts with regard to the ontogenesis and maturation of essential functions that take place over larval development. Our study is the first utilization of a transcriptomic approach in sea bass and reveals dynamic changes in gene expression patterns in relation to marine finfish larval development.
在幼体阶段，海洋硬骨鱼生长迅速，在形态、代谢和行为上发生显著变化，以完成向幼鱼的变态发育。基因表达调控被广泛认为是协调这一生命阶段和谐发育所需生物过程的关键机制。为了全面分析海鲈幼体发育过程中全身的基因表达情况，我们使用虹鳟鱼cDNA微阵列的异源杂交技术，在孵化后7至43天（dph）的10个不同时间点监测了6626个不同基因的表达。差异表达基因（n = 485）可分为两类：一类是在7至23 dph期间普遍早期上调表达的基因，另一类是在25至43 dph期间上调表达的基因。有趣的是，在幼体阶段受调控的基因中，与器官发生、能量途径、生物合成和消化相关的基因在分析的基因总数中占比过高。我们讨论了这些特定转录本在幼体发育过程中基本功能的个体发生和成熟方面的全身含量的定量调控。我们的研究是首次在海鲈中采用转录组学方法，揭示了与海洋硬骨鱼幼体发育相关的基因表达模式的动态变化。
