Kohler Annegret, Rinaldi Cécile, Duplessis Sébastien, Baucher Marie, Geelen Danny, Duchaussoy Frédéric, Meyers Blake C, Boerjan Wout, Martin Francis
Unité Mixte de Recherche INRA/UHP 1136 Interactions Arbres/Microorganismes, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, 54280 Champenoux, France.
Plant Mol Biol. 2008 Apr;66(6):619-36. doi: 10.1007/s11103-008-9293-9. Epub 2008 Feb 5.
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins ("NBS-LRR genes") play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of approximately 400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in rust-infected and non-infected leaves using a whole-genome oligoarray. None showed an altered expression two days post inoculation.
作为最大的一类抗病R基因，编码核苷酸结合位点和富含亮氨酸重复序列蛋白的基因（“NBS-LRR基因”）在保护植物免受多种病原体和害虫侵害方面发挥着关键作用。在毛果杨基因组草图序列中研究了NBS-LRR基因的多样性。这种多年生树木中的NBS类基因数量众多且种类多样，约有400个基因，至少是拟南芥中此类基因数量的两倍。NBS家族可分为多个具有不同结构域组织的亚家族。它包括119个卷曲螺旋-NBS-LRR基因、64个TIR-NBS-LRR基因、34个BED指状结构-NBS-LRR基因，以及截短的和不寻常的含NBS和NBS-LRR的基因。使用全基因组寡核苷酸芯片在感染锈病和未感染锈病的叶片中仅检测到34个NBS-LRR基因的转录本。接种两天后，没有一个显示出表达变化。
