Okulicz J F, Rivard R G, Conger N G, Nguyen M X, Hospenthal D R
Department of Infectious Diseases, Wilford Hall Medical Center, Lackland Air Force Base, San Antonio, TX, USA.
Mycoses. 2008 Mar;51(2):141-6. doi: 10.1111/j.1439-0507.2007.01456.x.
CHROMagar Candida (CaC) is a chromogenic medium that can be used to detect Candida species, including Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. We evaluated the utility of CaC to detect candiduria in high-risk patients and the potential usefulness of this information in directing initial antifungal therapy in those later identified with candidaemia. CaC was compared in parallel to standard laboratory methods (SM) for the detection of Candida from urine collected from high-risk units and wards. Of 893 samples, Candida was recovered by CaC from 104 compared with 35 using SM. No isolates detected by SM were undetected by CaC. More than one Candida species were recovered by CaC in 19 of the 104 (18.3%); only two mixed cultures were detected by SM. The identification was more rapid with CaC. Five of 69 patients with candiduria detected by CaC developed candidaemia on or after the date of urine culture. SM recovered fungus in only two of these patients. CaC can be used as primary media for the detection of Candida species from urine specimens. Primary isolation by CaC may enable clinicians to make earlier, directed selection of antifungal agents and potentially reduce patient morbidity and mortality.
科玛嘉念珠菌显色培养基（CHROMagar Candida，CaC）是一种显色培养基，可用于检测念珠菌属，包括白色念珠菌、克柔念珠菌、热带念珠菌，或许还能检测光滑念珠菌。我们评估了CaC在检测高危患者念珠菌尿方面的效用，以及该信息在指导后续确诊为念珠菌血症患者的初始抗真菌治疗中的潜在作用。将CaC与标准实验室方法（standard laboratory methods，SM）并行比较，以检测从高危科室和病房收集的尿液中的念珠菌。在893份样本中，CaC从104份样本中检出念珠菌，而SM仅从35份样本中检出。SM未检出的分离株，CaC也均未检出。CaC从104份样本中的19份（18.3%）检出了不止一种念珠菌；SM仅检测到两份混合培养物。CaC的鉴定速度更快。CaC检测出的69例念珠菌尿患者中，有5例在尿培养当天或之后发生了念珠菌血症。SM仅从其中两名患者中检出了真菌。CaC可作为从尿液标本中检测念珠菌属的主要培养基。通过CaC进行初步分离，可能使临床医生能够更早地、有针对性地选择抗真菌药物，并有可能降低患者的发病率和死亡率。
