使用显色培养基CHROMagar念珠菌对白色念珠菌、克柔念珠菌和热带念珠菌以外的念珠菌进行初步鉴定。
Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida.
作者信息
Hospenthal Duane R, Beckius Miriam L, Floyd Karon L, Horvath Lynn L, Murray Clinton K
机构信息
Department of Medicine, Brooke Army Medical Center, Fort Sam Houston, Texas, USA.
出版信息
Ann Clin Microbiol Antimicrob. 2006 Jan 3;5:1. doi: 10.1186/1476-0711-5-1.
BACKGROUND
CHROMagar Candida (CaC) is increasingly being reported as a medium used to differentiate Candida albicans from non-albicans Candida (NAC) species. Rapid identification of NAC can assist the clinician in selecting appropriate antifungal therapy. CaC is a differential chromogenic medium designed to identify C. albicans, C. krusei, and C. tropicalis based on colony color and morphology. Some reports have proposed that CaC can also reliably identify C. dubliniensis and C. glabrata.
METHODS
We evaluated the usefulness of CaC in the identification of C. dubliniensis, C. famata, C. firmetaria, C. glabrata, C. guilliermondii, C. inconspicua, C. kefyr, C. lipolytica, C. lusitaniae, C. norvegensis, C. parapsilosis, and C. rugosa.
RESULTS
Most NAC produced colonies that were shades of pink, lavender, or ivory. Several isolates of C. firmetaria and all C. inconspicua produced colonies difficult to differentiate from C. krusei. Most C. rugosa isolates produced unique colonies with morphology like C. krusei except in a light blue-green color. C. glabrata isolates produced small dark violet colonies that could be differentiated from the pink and lavender colors produced by other species. All seventeen isolates of C. dubliniensis produced green colonies similar to those produced by C. albicans.
CONCLUSION
C. glabrata and C. rugosa appear distinguishable from other species using CaC. Some NAC, including C. firmetaria and C. inconspicua, could be confused with C. krusei using this medium.
背景
CHROMagar念珠菌培养基（CaC）越来越多地被报道为一种用于区分白色念珠菌与非白色念珠菌（NAC）菌种的培养基。快速鉴定NAC有助于临床医生选择合适的抗真菌治疗方案。CaC是一种鉴别显色培养基，旨在根据菌落颜色和形态来鉴定白色念珠菌、克鲁斯念珠菌和热带念珠菌。一些报告提出CaC还能可靠地鉴定都柏林念珠菌和光滑念珠菌。
方法
我们评估了CaC在鉴定都柏林念珠菌、法塔念珠菌、坚固念珠菌、光滑念珠菌、季也蒙念珠菌、隐匿念珠菌、凯菲念珠菌、解脂念珠菌、葡萄牙念珠菌、挪威念珠菌、近平滑念珠菌和皱落念珠菌中的实用性。
结果
大多数NAC产生的菌落为粉色、淡紫色或象牙色。几株坚固念珠菌和所有隐匿念珠菌产生的菌落难以与克鲁斯念珠菌区分。大多数皱落念珠菌分离株产生的独特菌落形态与克鲁斯念珠菌相似，只是颜色为浅蓝绿色。光滑念珠菌分离株产生小的深紫色菌落，可与其他菌种产生的粉色和淡紫色菌落区分开来。所有17株都柏林念珠菌产生的绿色菌落与白色念珠菌产生的菌落相似。
结论
使用CaC，光滑念珠菌和皱落念珠菌似乎可与其他菌种区分开来。使用这种培养基，一些NAC，包括坚固念珠菌和隐匿念珠菌，可能会与克鲁斯念珠菌混淆。
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