Garbuzynskiy S O, Kondratova M S
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
FEBS Lett. 2008 Mar 5;582(5):768-72. doi: 10.1016/j.febslet.2008.01.049. Epub 2008 Feb 5.
A crucial event of protein folding is the formation of a folding nucleus. We demonstrate the presence of a considerable coincidence between the location of folding nuclei and the location of so-called "root structural motifs", which have unique overall folds and handedness. In the case of proteins with a single root structural motif, the involvement in the formation of a folding nucleus is in average significantly higher for amino acids residues that are in root structural motifs, compared to residues in other parts of the protein. The tests carried out revealed that the observed difference is statistically reliable. Thus, a structural feature that corresponds to the protein folding nucleus is now found.
蛋白质折叠的一个关键事件是折叠核的形成。我们证明了折叠核的位置与所谓“根结构基序”的位置之间存在相当大的一致性，根结构基序具有独特的整体折叠和手性。对于具有单个根结构基序的蛋白质，与蛋白质其他部分的残基相比，根结构基序中的氨基酸残基参与折叠核形成的平均程度明显更高。所进行的测试表明，观察到的差异在统计学上是可靠的。因此，现在发现了一种与蛋白质折叠核相对应的结构特征。
