Julsing Mattijs K, Cornelissen Sjef, Bühler Bruno, Schmid Andreas
Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Strasse 66, D-44221 Dortmund, Germany.
Curr Opin Chem Biol. 2008 Apr;12(2):177-86. doi: 10.1016/j.cbpa.2008.01.029. Epub 2008 Mar 4.
Are cytochrome P450 enzymes powerful industrial biocatalysts? Next to market demands, well-defined enzyme functionalities and process parameters allow generalizations on the basis of process windows. These can provide useful guidelines for the design of improved biocatalysts. Oxygenase-catalyzed reactions are of special interest for selective C-H bond oxidation. The versatile class of cytochrome P450 mono-oxygenases attracts particular attention, and impressive advances have been achieved with respect to mechanistic insight, enzyme activity, stability, and specificity. Recent major achievements include significant increases in productivities, yields, and rates of catalytic turnover as well as modification of substrate specificity and efficient multistep reactions in whole-cell biocatalysts. For some biocatalysts, these parameters are already of an industrially useful magnitude.
细胞色素P450酶是强大的工业生物催化剂吗？除了市场需求外，明确的酶功能和工艺参数允许在工艺窗口的基础上进行概括。这些可为改进型生物催化剂的设计提供有用的指导方针。加氧酶催化的反应对于选择性C-H键氧化特别有意义。多功能的细胞色素P450单加氧酶类别引起了特别关注，并且在机理洞察、酶活性、稳定性和特异性方面已经取得了令人瞩目的进展。最近的主要成就包括生产率、产率和催化周转率的显著提高，以及底物特异性的改变和全细胞生物催化剂中的高效多步反应。对于一些生物催化剂来说，这些参数已经达到了工业上有用的量级。
