Llewellyn S, Fitzpatrick R, Kenny D A, Patton J, Wathes D C
Reproduction, Genes and Development Group, Department of Veterinary Basic Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield, Herts, London AL9 7TA, UK.
Domest Anim Endocrinol. 2008 May;34(4):391-402. doi: 10.1016/j.domaniend.2007.11.003. Epub 2007 Dec 26.
Rapid uterine involution in the postpartum period of dairy cows is important to achieve a short interval to conception. Expression patterns for members of the insulin-like growth factor (IGF) family were determined by in situ hybridisation at day 14+/-0.4 postpartum (n=12 cows) to investigate a potential role for IGFs in modulating uterine involution. Expression in each uterine tissue region was measured as optical density units and data were analysed according to region and horn. IGF-I mRNA was localized to the sub-epithelial stroma (SES) of inter-caruncular and caruncular endometrium. Both IGF-II and IGF-1R expression was detected in the deep endometrial stroma (DES), the caruncular stroma and myometrium. IGFBP-2, IGFBP-4 and IGFBP-6 mRNAs were all localised to the SES of inter-caruncular and caruncular uterine tissue, and in the DES and caruncular stroma, with IGFBP-4 mRNA additionally expressed in myometrium. IGFBP-3 mRNA was only detectable in luminal epithelium. IGFBP-5 mRNA was found in myometrium, inter-caruncular and caruncular SES and caruncular stroma. These data support a role for IGF-I and IGF-II in the extensive tissue remodelling and repair which the postpartum uterus undergoes to return to its non-pregnant state. The differential expression of binding proteins between tissues (IGFBP-3 in epithelium, IGFBP-2, -4, -5 and -6 in stroma and IGFBP-4 and -5 in myometrium) suggest tight control of IGF activity within each compartment. Differential expression of many members of the IGF family between the significantly larger previously gravid horn and the previously non-gravid horn may relate to differences in their rate of tissue remodelling.
奶牛产后子宫快速复旧对于缩短受孕间隔至关重要。通过原位杂交技术在产后14±0.4天（n = 12头奶牛）测定胰岛素样生长因子（IGF）家族成员的表达模式，以研究IGF在调节子宫复旧中的潜在作用。每个子宫组织区域的表达以光密度单位进行测量，并根据区域和子宫角对数据进行分析。IGF-I mRNA定位于肉阜间和肉阜子宫内膜的上皮下基质（SES）。IGF-II和IGF-1R的表达均在深层子宫内膜基质（DES）、肉阜基质和肌层中检测到。IGFBP-2、IGFBP-4和IGFBP-6 mRNA均定位于肉阜间和肉阜子宫组织的SES，以及DES和肉阜基质中，其中IGFBP-4 mRNA还在肌层中表达。IGFBP-3 mRNA仅在腔上皮中可检测到。IGFBP-5 mRNA在肌层、肉阜间和肉阜SES以及肉阜基质中被发现。这些数据支持IGF-I和IGF-II在产后子宫为恢复到非妊娠状态而进行的广泛组织重塑和修复中发挥作用。各组织间结合蛋白的差异表达（上皮中的IGFBP-3、基质中的IGFBP-2、-4、-5和-6以及肌层中的IGFBP-4和-5）表明每个隔室内IGF活性受到严格控制。IGF家族许多成员在明显更大的先前妊娠子宫角和先前未妊娠子宫角之间的差异表达可能与其组织重塑速率的差异有关。
