Ameglio F, Capobianchi M R, Castilletti C, Cordiali Fei P, Fais S, Trento E, Dianzani F
Institute S. Gallicano, University La Sapienza, Rome, Italy.
Clin Exp Immunol. 1994 Mar;95(3):455-8. doi: 10.1111/j.1365-2249.1994.tb07018.x.
Immunological abnormalities present in HIV-1-infected individuals often reflect an imbalance of cytokine production. The HIV-1 gp120 has the ability to induce a number of cytokines, and to enhance immunoglobulin release by normal peripheral blood mononuclear cells (PBMC) in vitro, in the absence of IL-2 production and of lymphoproliferation. This study provides evidence that gp120 is a potent IL-10 inducer in normal PBMC cultures. The pattern of other cytokines induced by gp120 includes interferon-alpha (IFN-alpha) and IFN-gamma, tumour necrosis factor-alpha (TNF-alpha), IL-6, IL-1 alpha and -beta, and not IL-2 and IL-4. These findings further define the pattern of cytokine release induced by gp120 on human resting PBMC. Furthermore, the present findings roughly parallel those observed both in the sera of patients and in the mononuclear cells from HIV+ individuals early after infection, suggesting that gp120 could be a good candidate as one of the agents responsible for cytokine dysregulation observed in HIV-1-infected individuals.
HIV-1感染个体中出现的免疫异常往往反映了细胞因子产生的失衡。HIV-1 gp120能够诱导多种细胞因子，并在体外增强正常外周血单个核细胞（PBMC）释放免疫球蛋白，同时不产生IL-2且无淋巴细胞增殖。本研究提供了证据表明gp120是正常PBMC培养物中一种有效的IL-10诱导剂。gp120诱导的其他细胞因子模式包括α干扰素（IFN-α）和γ干扰素、肿瘤坏死因子-α（TNF-α）、IL-6、IL-1α和-β，而不包括IL-2和IL-4。这些发现进一步明确了gp120对人静息PBMC诱导的细胞因子释放模式。此外，目前的发现与在感染早期患者血清和HIV+个体的单个核细胞中观察到的情况大致相似，这表明gp120可能是导致HIV-1感染个体中观察到的细胞因子失调的因素之一的良好候选者。
