Pancic P G, Strotmann H, Kowallik K V
Institut für Biochemie, Pflanzen, Düsseldorf, Germany.
FEBS Lett. 1991 Mar 25;280(2):387-92. doi: 10.1016/0014-5793(91)80338-4.
A 5.2 kb PstI restriction fragment containing the atpA gene cluster of the plastic genome of the centric diatom Odontella sinensis was cloned. Sequencing revealed a reading frame of 561 bp separating the genes atpF and atpA, which is preceded by a putative ribosome binding site. The third nucleotide of the codon for the last amino acid of atpF is the first nucleotide of the initiation codon of the 561 bp reading frame. The amino acid sequence deduced from the nucleotide sequence of this gene (atpD) is colinear with delta subunits of different F0F1-ATPases and shows an overall sequence homology of up to 35% when compared with the sequences of cyanobacteria and Cyanophora paradoxa. The results are discussed in context with the evolution of chloroplasts of the chlorophyll-a + b and -a + c lineages, respectively.
克隆了一个包含中心硅藻中华齿状藻质体基因组atpA基因簇的5.2 kb PstI限制性片段。测序显示有一个561 bp的阅读框将atpF和atpA基因隔开，该阅读框之前有一个假定的核糖体结合位点。atpF最后一个氨基酸密码子的第三个核苷酸是561 bp阅读框起始密码子的第一个核苷酸。从该基因（atpD）的核苷酸序列推导的氨基酸序列与不同F0F1 - ATP酶的δ亚基共线性，与蓝细菌和奇异蓝杆藻的序列相比，整体序列同源性高达35%。分别结合叶绿素 - a + b和 - a + c谱系叶绿体的进化对结果进行了讨论。
