Becker D M, Lundblad V
Stanford Law School, Stanford, California, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 13:Unit13.7. doi: 10.1002/0471142727.mb1307s27.
The most commonly used yeast transformation protocol is the lithium acetate procedure (described here). It is reasonably fast and provides a transformation efficiency of 10(5) to 10(6) transformants/microg. This efficiency rivals that achieved for most, but not all, strains with the more difficult and time-consuming spheroplast procedure presented here. However, the fastest and easiest of the transformation methods is electroporation, as described in this unit. For a number of strains, electroporation offers the highest transformation efficiency, and may prove especially useful with limiting quantities of transforming DNA. Unlike the lithium acetate procedure, however, electroporation saturates at low DNA levels, restricting its general utility.
最常用的酵母转化方案是醋酸锂法（在此处描述）。它相当快速，转化效率为每微克10⁵至10⁶个转化子。对于大多数（但不是所有）菌株而言，这种效率与此处介绍的更困难且耗时的原生质球法所达到的效率相当。然而，本单元所述的最快且最简单的转化方法是电穿孔法。对于许多菌株来说，电穿孔法提供了最高的转化效率，并且在转化DNA量有限时可能特别有用。然而，与醋酸锂法不同，电穿孔法在低DNA水平时会达到饱和，限制了其通用性。
