Neely R D, Kiwanuka J B, Hadden D R
Department of Clinical Biochemistry, Royal Victoria Hospital, Belfast, UK.
Diabet Med. 1991 Feb-Mar;8(2):129-34. doi: 10.1111/j.1464-5491.1991.tb01558.x.
The World Health Organization (1985) criteria allow evaluation of the oral glucose tolerance test using venous or capillary whole blood or plasma glucose measurements. However, the empirical factors used for interconversion may not reflect observed differences, especially during pregnancy, causing inconsistent classification. To investigate how choice of sample would influence the interpretation of results, venous and capillary blood was taken during oral glucose tolerance tests in 36 pregnant women at risk of gestational diabetes and in 21 non-pregnant control subjects. Glucose was measured on whole blood and plasma by a glucose oxidase method. No cases of gestational diabetes were identified. Eight subjects had gestational Impaired Glucose Tolerance using either venous plasma or venous whole blood results, but only five were similarly classified with capillary whole blood and only four using capillary plasma. Plasma-whole blood differences (venous 0.6 +/- 0.2 (+/- SD) mmol l-1, capillary 0.7 +/- 0.3 mmol l-1) and capillary-venous differences (plasma 0.5 +/- 0.4, whole blood 0.4 +/- 0.5 mmol l-1) at 2 h were lower (all p less than 0.05) than in the WHO criteria (1.1 mmol l-1). When compared with venous plasma, capillary measurements may give a lower incidence and venous whole blood measurements a higher incidence of Impaired Glucose Tolerance in pregnancy.
世界卫生组织（1985年）的标准允许使用静脉血或毛细血管全血或血浆葡萄糖测量值来评估口服葡萄糖耐量试验。然而，用于相互转换的经验性因素可能无法反映观察到的差异，尤其是在孕期，这会导致分类不一致。为了研究样本选择如何影响结果的解读，在36名有妊娠期糖尿病风险的孕妇和21名非孕对照受试者进行口服葡萄糖耐量试验期间采集了静脉血和毛细血管血。通过葡萄糖氧化酶法对全血和血浆中的葡萄糖进行测量。未发现妊娠期糖尿病病例。使用静脉血浆或静脉全血结果时，有8名受试者存在妊娠期糖耐量受损，但使用毛细血管全血时只有5名受试者被同样分类，使用毛细血管血浆时只有4名受试者被同样分类。2小时时的血浆与全血差异（静脉血0.6±0.2（±标准差）mmol/L，毛细血管血0.7±0.3 mmol/L）以及毛细血管血与静脉血差异（血浆0.5±0.4，全血0.4±0.5 mmol/L）低于世界卫生组织标准中的差异（1.1 mmol/L）（所有p均小于0.05）。与静脉血浆相比，毛细血管测量可能会使孕期糖耐量受损的发生率降低，而静脉全血测量则会使其发生率升高。
