Chantret Nathalie, Salse Jérôme, Sabot François, Bellec Arnaud, Laubin Bastien, Dubois Ivan, Dossat Carole, Sourdille Pierre, Joudrier Philippe, Gautier Marie-Françoise, Cattolico Laurence, Beckert Michel, Aubourg Sébastien, Weissenbach Jean, Caboche Michel, Leroy Philippe, Bernard Michel, Chalhoub Boulos
Domaine de Melgueil, INRA-UMR DIAPC, 34130 Mauguio, France.
J Mol Evol. 2008 Feb;66(2):138-50. doi: 10.1007/s00239-008-9066-8. Epub 2008 Feb 15.
We study here the evolution of genes located in the same physical locus using the recently sequenced Ha locus in seven wheat genomes in diploid, tetraploid, and hexaploid species and compared them with barley and rice orthologous regions. We investigated both the conservation of microcolinearity and the molecular evolution of genes, including coding and noncoding sequences. Microcolinearity is restricted to two groups of genes (Unknown gene-2, VAMP, BGGP, Gsp-1, and Unknown gene-8 surrounded by several copies of ATPase), almost conserved in rice and barley, but in a different relative position. Highly conserved genes between wheat and rice run along with genes harboring different copy numbers and highly variable sequences between close wheat genomes. The coding sequence evolution appeared to be submitted to heterogeneous selective pressure and intronic sequences analysis revealed that the molecular clock hypothesis is violated in most cases.
我们利用最近测序的七个二倍体、四倍体和六倍体小麦基因组中的Ha基因座，研究了位于同一物理位点的基因的进化，并将它们与大麦和水稻的直系同源区域进行了比较。我们研究了微共线性的保守性以及基因的分子进化，包括编码和非编码序列。微共线性仅限于两组基因（未知基因-2、VAMP、BGGP、Gsp-1和未知基因-8，周围有几个ATPase拷贝），在水稻和大麦中几乎是保守的，但相对位置不同。小麦和水稻之间高度保守的基因与具有不同拷贝数的基因以及亲缘关系较近的小麦基因组之间高度可变的序列一起存在。编码序列的进化似乎受到异质选择压力的影响，内含子序列分析表明，在大多数情况下，分子钟假说是不成立的。
