GM1表位四糖的高效构建及其与钥孔血蓝蛋白的偶联
A highly efficient construction of GM1 epitope tetrasaccharide and its conjugation with KLH.
作者信息
Yoshikawa Takeru, Kato Yuki, Yuki Nobuhiro, Yabe Tomio, Ishida Hideharu, Kiso Makoto
机构信息
Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan.
出版信息
Glycoconj J. 2008 Aug;25(6):545-53. doi: 10.1007/s10719-008-9111-2. Epub 2008 Feb 15.
PMID:18274892
Abstract
GM1 epitope tetrasaccharide was synthesized by a condensation of sialyl-alpha(2-3)-gal acceptor and gal-beta(1-3)-GalN donor in a highly efficient manner. After introduction of mercaptohexanol to the tetrasaccharide, it was coupled to maleimide-activated KLH carrier protein to give the desired GM1 epitope-KLH conjugate.
摘要
GM1表位四糖通过唾液酸-α(2-3)-半乳糖受体与半乳糖-β(1-3)-氨基半乳糖供体高效缩合合成。在四糖中引入巯基己醇后，将其与马来酰亚胺活化的钥孔血蓝蛋白（KLH）载体蛋白偶联，得到所需的GM1表位-KLH缀合物。
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