Alonso de Velasco E, Verheul A F, Veeneman G H, Gomes L J, van Boom J H, Verhoef J, Snippe H
Eijkman-Winkler Laboratory of Medical Microbiology, Utrecht University, The Netherlands.
Vaccine. 1993 Nov;11(14):1429-36. doi: 10.1016/0264-410x(93)90172-t.
Overlapping synthetic disaccharide, trisaccharide and tetrasaccharide, derived from pneumococcal polysaccharide type 17F (PS17F), were coupled to keyhole limpet haemocyanin (KLH). The conjugates were tested in mice. The disaccharide-KLH and especially trisaccharide-KLH, in combination with Quil A, induced high titres of high-avidity anti-PS17F IgG. Both conjugates protected mice against challenge with Streptococcus pneumoniae 17F. Tetrasaccharide-KLH, although able to elicit anti-tetrasaccharide antibodies, induced a minimal non-protective anti-PS17F IgG response of low avidity. The tetrasaccharide-KLH conjugate, in contrast to the other conjugates, failed to bind rabbit anti-PS17F IgG.
源自17F型肺炎球菌多糖（PS17F）的重叠合成二糖、三糖和四糖与钥孔戚血蓝蛋白（KLH）偶联。在小鼠中对这些偶联物进行了测试。二糖-KLH，尤其是三糖-KLH，与Quil A联合使用时，可诱导产生高滴度的高亲和力抗PS17F IgG。两种偶联物均能保护小鼠免受17F型肺炎链球菌的攻击。四糖-KLH虽然能够引发抗四糖抗体，但诱导出的是低亲和力的最小非保护性抗PS17F IgG反应。与其他偶联物相比，四糖-KLH偶联物无法结合兔抗PS17F IgG。
