Reynolds Clare M, Loscher Christine E, Moloney Aidan P, Roche Helen M
Nutrigenomics Research Group, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland.
Br J Nutr. 2008 Jul;100(1):13-7. doi: 10.1017/S0007114508894329.
Trans-vaccenic acid (TVA) is a natural trans fatty acid found in ruminant food produce. It is converted to the cis-9, trans-11 isomer of conjugated linoleic acid (c9, t11-CLA) by the action of stearoyl-CoA desaturase (SCD) in tissue. c9, t11-CLA has been associated with anti-inflammatory effects and also affects lipid metabolism. The aim of the present study was to determine if TVA is bioconverted to c9, t11-CLA in intestinal epithelial cells and to ascertain whether TVA has effects similar to c9, t11-CLA on markers of inflammation relevant to inflammatory bowel disease. The present study demonstrated that TVA treatment led to significant bioconversion into c9, t11-CLA in Caco-2 cells. Treatment with both TVA and c9, t11-CLA resulted in alteration of cellular fatty acid profile and SCD activity in the Caco-2 cell line. However, CLA, but not TVA, significantly modulated transcription of TNF-alpha, IL-12, IL-6 and production of IL-12 by these cells. Thus the present study established that TVA treatment can alter SCD desaturation indices and induce compositional changes in the fatty acid profile of the Caco-2 cell model of the human intestinal epithelium but this is not associated with functional effects on markers of the inflammatory response.
反式vaccenic酸（TVA）是一种存在于反刍动物食品中的天然反式脂肪酸。它在组织中通过硬脂酰辅酶A去饱和酶（SCD）的作用转化为共轭亚油酸的顺-9，反-11异构体（c9，t11-CLA）。c9，t11-CLA具有抗炎作用，还影响脂质代谢。本研究的目的是确定TVA在肠上皮细胞中是否会生物转化为c9，t11-CLA，并确定TVA是否具有与c9，t11-CLA类似的对与炎症性肠病相关的炎症标志物的影响。本研究表明，TVA处理导致Caco-2细胞中显著生物转化为c9，t11-CLA。用TVA和c9，t11-CLA处理均导致Caco-2细胞系中细胞脂肪酸谱和SCD活性的改变。然而，CLA而非TVA显著调节这些细胞中TNF-α、IL-12、IL-6的转录以及IL-12的产生。因此，本研究确定，TVA处理可改变SCD去饱和指数并诱导人肠上皮细胞Caco-2细胞模型脂肪酸谱的组成变化，但这与对炎症反应标志物的功能影响无关。
