Farage Miranda A
Feminine Clinical Sciences, The Procter & Gamble Company, Cincinnati, OH 45224, USA.
Contact Dermatitis. 2008 Mar;58(3):147-55. doi: 10.1111/j.1600-0536.2007.01284.x.
BACKGROUND/PURPOSE: Polarized light has been used as an aid in visualizing various skin conditions, including acne vulgaris, rosacea, photoageing, lentigo simplex, and basal cell carcinoma. The use of parallel-polarized and cross-polarized light was evaluated in mild irritant reactions to determine, if this increases the ability to detect very early stages or low levels of irritation.
Low concentrations of sodium lauryl sulfate (0.01% and 0.1%) were patched on human volunteers for 2, 6, and 24 hr, daily for 2-3 days in a modification of the standard patch test. Feminine protection products were evaluated in the behind-the-knee (BTK) test. Erythema reactions were scored by unaided visual assessment and using a polarized light visualization system.
In the 24-hr patch test, mean erythema assessed with polarized light was consistent with results of unaided visual scoring. Under milder conditions (2- and 6-hr patches), and in the BTK, significant differences from pretreatment levels of erythema were apparent earlier in the series of treatments compared with unaided scoring. In addition, subsurface scoring demonstrated that changes were still present under the skin surface even after unaided visual scoring indicated recovery.
Low (subclinical) levels of irritation can be detected using enhanced visual scoring, indicating this non-invasive method has the potential to increase the sensitivity of our clinical studies.
背景/目的：偏振光已被用于辅助观察各种皮肤状况，包括寻常痤疮、玫瑰痤疮、光老化、单纯性雀斑样痣和基底细胞癌。本研究评估了平行偏振光和交叉偏振光在轻度刺激性反应中的应用，以确定其是否能提高检测极早期或低水平刺激的能力。
在标准斑贴试验的基础上进行改良，将低浓度的月桂醇硫酸酯钠（0.01%和0.1%）贴敷于人类志愿者身上2、6和24小时，每天一次，持续2至3天。在膝后（BTK）试验中评估女性护理产品。通过肉眼视觉评估和使用偏振光可视化系统对红斑反应进行评分。
在24小时斑贴试验中，用偏振光评估的平均红斑与肉眼视觉评分结果一致。在较温和的条件下（2小时和6小时贴敷）以及在BTK试验中，与肉眼评分相比，在一系列治疗中红斑与治疗前水平的显著差异出现得更早。此外，皮下评分显示，即使肉眼视觉评分表明已恢复，皮肤表面下仍存在变化。
使用增强视觉评分可以检测到低（亚临床）水平的刺激，表明这种非侵入性方法有可能提高我们临床研究的敏感性。
