Mantzaris G, Jewell D P
Gastroenterology Unit, Radcliffe Infirmary, Oxford, U.K.
Scand J Gastroenterol. 1991 Apr;26(4):392-8. doi: 10.3109/00365529108996500.
A single dose of a synthetic peptide of A gliadin (residues 206-217) sharing homology with the E1b protein of adenovirus 12 was instilled intraduodenally in two treated coeliac patients. Biopsy specimens were taken before and repeatedly up to 24 h after the instillation by means of a Quinton hydraulic multiple biopsy instrument and processed for histology, morphometry (intraepithelial lymphocyte counts, crypt-to-villus ratio), immunocytochemistry, electron microscopy, and disaccharidase assays. Two subjects with irritable bowel syndrome served as controls. In the coeliac group disaccharidase activities decreased at 24 h, and abnormalities were seen on light and electron microscopy and in morphometric measurements. The lamina propria became infiltrated with mononuclear cells after 2 h, and there was also a rise in IgA-containing cells in one patient. No such abnormalities were seen in the control group. The serum concentrations of C3, C4, and C1 esterase inhibitor remained unchanged. Thus, the dodecapeptide may be one epitope of gliadin mediating the pathogenesis of coeliac disease.
将与腺病毒12的E1b蛋白具有同源性的麦醇溶蛋白合成肽（残基206 - 217）的单剂量经十二指肠内注入两名接受治疗的乳糜泻患者体内。在注入前以及注入后长达24小时内，通过昆顿液压式多部位活检器械反复采集活检标本，并进行组织学、形态计量学（上皮内淋巴细胞计数、隐窝与绒毛比例）、免疫细胞化学、电子显微镜检查以及双糖酶测定。两名肠易激综合征患者作为对照。在乳糜泻组中，双糖酶活性在24小时时下降，并且在光学显微镜和电子显微镜检查以及形态计量学测量中均出现异常。2小时后固有层出现单核细胞浸润，并且在一名患者中含IgA细胞也有所增加。对照组未观察到此类异常。血清C3、C4和C1酯酶抑制剂浓度保持不变。因此，该十二肽可能是介导乳糜泻发病机制的麦醇溶蛋白的一个表位。
