Cosentino Marco, Bombelli Raffaella, Carcano Elena, Luini Alessandra, Marino Franca, Crema Francesca, Dajas Federico, Lecchini Sergio
Department of Clinical Medicine, University of Insubria, Varese, Italy.
J Ethnopharmacol. 2008 Mar 28;116(3):501-7. doi: 10.1016/j.jep.2007.12.014. Epub 2008 Jan 6.
Achyrocline satureioides (Lam.) D.C. is a South American native medicinal herb known by the popular name of "Marcela". Its infusion is widely utilized for the treatment of several digestive ailments, as an anti-inflammatory preparation, as a sedative and anti-atherosclerotic. Circumstantial evidence suggests that extracts of Achyrocline satureioides may have immunomodulatory properties. The present study was therefore devised to investigate the in vitro effects Achyrocline satureioides infusion on human peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs).
Experiments were performed on cells isolated from venous blood obtained from healthy donors. PBMC proliferation and cytokine production were assessed by standard ELISA methods. Reactive oxygen species (ROS) production by PMNs was evaluated by spectrofluorimetry.
In PBMCs, Achyrocline satureioides infusion in the 0.06-0.24microg/ml quercetin equivalent (QE) concentration range concentration-dependently reduced PHA-induced proliferation and production of interferon (IFN)-gamma and interleukin (IL)-4. Lower concentrations of the infusion (0.006-0.03microg/ml QE), which were ineffective on cell proliferation, significantly increased the production of both IFN-gamma and IL-4 and decreased the ratio IFN-gamma/IL-4. In PMNs, Achyrocline satureioides infusion slightly increased the spontaneous generation of ROS only at concentrations > or =0.06microg/ml QE. On the contrary, in the 0.0012-0.03microg/ml QE concentration range the infusion profoundly inhibited fMLP-induced ROS generation as well as spontaneous and fMLP-induced IL-8 production.
The present results provide evidence that Achyrocline satureioides infusion may exert several immunomodulatory effects, in line with its traditional use as an anti-inflammatory agent in many disease conditions. Further studies are warranted to better characterize such effects and to assess their therapeutic relevance.
马齿笕（Achyrocline satureioides (Lam.) D.C.）是一种原产于南美的药草，俗名“Marcela”。其浸剂被广泛用于治疗多种消化系统疾病，作为抗炎制剂、镇静剂和抗动脉粥样硬化药物。间接证据表明，马齿笕提取物可能具有免疫调节特性。因此，本研究旨在探讨马齿笕浸剂对人外周血单个核细胞（PBMCs）和多形核白细胞（PMNs）的体外作用。
实验采用从健康供体静脉血中分离的细胞进行。通过标准ELISA方法评估PBMC增殖和细胞因子产生。通过荧光分光光度法评估PMNs产生的活性氧（ROS）。
在PBMCs中，浓度范围为0.06 - 0.24微克/毫升槲皮素当量（QE）的马齿笕浸剂浓度依赖性地降低了PHA诱导的增殖以及干扰素（IFN）-γ和白细胞介素（IL）-4的产生。浸剂较低浓度（0.006 - 0.03微克/毫升QE）对细胞增殖无效，但显著增加了IFN-γ和IL-4的产生，并降低了IFN-γ/IL-4的比值。在PMNs中，仅当浓度≥0.06微克/毫升QE时，马齿笕浸剂才会轻微增加ROS的自发产生。相反，在0.0012 - 0.03微克/毫升QE浓度范围内，浸剂显著抑制了fMLP诱导的ROS产生以及自发和fMLP诱导的IL-8产生。
目前的结果提供了证据表明马齿笕浸剂可能发挥多种免疫调节作用，这与其在许多疾病状态下作为抗炎剂使用的传统用途一致。有必要进行进一步研究以更好地表征这些作用并评估其治疗相关性。
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