Caldefie-Chézet F, Fusillier C, Jarde T, Laroye H, Damez M, Vasson M-P, Guillot J
Laboratoire de Botanique, Cryptogamie et Microbiologie, CRNH Auvergne, Clermont-Ferrand, France.
Phytother Res. 2006 May;20(5):364-70. doi: 10.1002/ptr.1862.
The fungicidal and bactericidal actions of the essential oil (EO) of Melaleuca alternifolia seem well established, but their anti-inflammatory and antioxidative effects remain unclear. This study investigated in vitro the possible role of whole Melaleuca alternifolia EO as a modulator of the inflammatory/non-specific immune response by exploring the chemotaxis and kinetic radical oxygen species (ROS) production of leukocytes and cytokine secretion in peripheral blood mononuclear cells (PBMCs) in humans. The influence of Melaleuca alternifolia EO on the chemotaxis under agarose of isolated neutrophils (PMNs) was evaluated. The kinetics of ROS production by stimulated total circulating leukocytes was followed over 2 h by recording the fluorescence intensity of oxidized dihydrorhodamine 123. The effects of this EO on pro-(interleukin IL-2) and anti-(IL-4 and IL10) inflammatory cytokine secretions were determined by ELISA following incubation of PBMCs with the EO for 24 h. Melaleuca alternifolia EO was inefficient on the chemotaxis of PMNs. It exerted an antioxidant effect, reducing ROS production throughout the kinetic study. Melaleuca alternifolia EO inhibited PBMC proliferation, as revealed by a reduction in IL-2 secretion by stimulated lymphocytes. This EO at 0.1% directly increased the secretion of the anti-inflammatory cytokine IL-4 compared with IL-4 secretion without EO (18.5 +/- 10.0 vs 3.3 +/- 1, p < 0.05), and also increased IL-10 secretion at 0.01% (94.9 +/- 38.7 vs 44.1 +/- 18, ns). Melaleuca alternifolia EO may not only act as an anti-inflammatory mediator through its antioxidant activity but may also efficiently protect the organism by reducing the proliferation of inflammatory cells without affecting their capacity to secrete anti-inflammatory cytokines.
互叶白千层精油（EO）的杀菌作用似乎已得到充分证实，但其抗炎和抗氧化作用仍不明确。本研究通过探讨人体外周血单个核细胞（PBMCs）中白细胞的趋化性、活性氧（ROS）生成动力学以及细胞因子分泌情况，在体外研究了互叶白千层全株EO作为炎症/非特异性免疫反应调节剂的潜在作用。评估了互叶白千层EO对分离的中性粒细胞（PMNs）在琼脂糖下趋化性的影响。通过记录氧化二氢罗丹明123的荧光强度，跟踪刺激的全循环白细胞产生ROS的动力学过程，持续2小时。在PBMCs与EO孵育24小时后，通过酶联免疫吸附测定（ELISA）确定该EO对促炎细胞因子（白细胞介素IL - 2）和抗炎细胞因子（IL - 4和IL10）分泌的影响。互叶白千层EO对PMNs的趋化性作用不佳。在整个动力学研究中，它发挥了抗氧化作用，减少了ROS的产生。互叶白千层EO抑制了PBMC的增殖，这通过刺激淋巴细胞分泌IL - 2减少得以体现。与无EO时的IL - 4分泌相比（18.5±10.0对3.3±1，p<0.05），0.1%的该EO直接增加了抗炎细胞因子IL - 4的分泌，并且在0.01%时也增加了IL - 10的分泌（94.9±38.7对44.1±18，无显著差异）。互叶白千层EO不仅可能通过其抗氧化活性作为抗炎介质发挥作用，还可能通过减少炎症细胞的增殖而不影响其分泌抗炎细胞因子的能力来有效保护机体。
