Rivero V E, Ferro M E, Romero-Piffiguer M, Correa S, Yranzo-Volonté N, Riera C M
Inmunologia, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
Eur J Immunol. 1991 May;21(5):1141-6. doi: 10.1002/eji.1830210508.
The present report describes different aspects of two populations of peritoneal cells (PC) obtained from rats injected i.p. 2 h or 24 h previously with a suppressor dose of a purified fraction (FI) of rat male accessory glands (RAG) (FI-PC2h and FI-PC24h, respectively). The FI-PC2h, which are mainly I-E (OX17) positive and can suppress the autoimmune response to RAG autoantigens, have an elevated phagocytic activity against Candida albicans and capacity to reduce the dye nitroblue tetrazolium. In contrast, FI-PC24h, which are mainly I-A (OX6) positive and can potentiate the autoimmunity to RAG autoantigens, have a diminished capacity to reduce the dye and a diminished phagocytic activity. Moreover, the Toxoplasma gondii appear to have a different effect on both populations. The parasites can invade FI-PC2h while FI-PC24h offer resistance to T. gondii aggression. FI-PC2h cultured during 22 h (FI-PC2-24h in vitro), or PC obtained from syngeneic recipients injected i.p. 22 h previously with FI-PC2h (FI-PC2-24h in vivo) show, as FI-PC2h, an increase of the I-E+ cells and capacity to induce suppression of the delayed-type hypersensitivity response to RAG autoantigens when they are injected to syngeneic rats 10 and 3 days prior to the immunization with chemically modified (diazotized arsanilic and sulfanilic acid) RAG in complete Freund's adjuvant. The PC obtained 24 h after injection of irradiated rats with N-PC plus FI show an increase of I-E+ cells whereas an enhancement of I-A+ cells can be observed when the PC are obtained 24 h after injection of irradiated and bone marrow-reconstituted rats with N-PC plus FI. These findings appear to indicate that FI-PC2h and FI-PC24h are functionally different and that the population obtained 24 h after injection of FI of RAG could not originate from either the population present 2 h after injection of FI of RAG injection nor from normal PC. They appear to require bone marrow precursors.
本报告描述了从腹腔注射过纯化的大鼠雄性附属性腺（RAG）组分（FI）（抑制剂量）2小时或24小时的大鼠获得的两类腹膜细胞（PC）的不同方面（分别为FI-PC2h和FI-PC24h）。FI-PC2h主要为I-E（OX17）阳性，能够抑制对RAG自身抗原的自身免疫反应，对白色念珠菌具有增强的吞噬活性，并且具有还原染料硝基蓝四氮唑的能力。相比之下，FI-PC24h主要为I-A（OX6）阳性，能够增强对RAG自身抗原的自身免疫性，还原染料的能力减弱，吞噬活性也减弱。此外，刚地弓形虫对这两类细胞似乎有不同的作用。寄生虫能够侵入FI-PC2h，而FI-PC24h对刚地弓形虫的侵袭具有抗性。培养22小时的FI-PC2h（体外FI-PC2-24h），或从22小时前腹腔注射过FI-PC2h的同基因受体获得的PC（体内FI-PC2-24h），与FI-PC2h一样，当在化学修饰（重氮化对氨基苯胂酸和对氨基苯磺酸）的RAG与完全弗氏佐剂免疫前10天和3天注射给同基因大鼠时，I-E +细胞增加，并且具有诱导抑制对RAG自身抗原的迟发型超敏反应的能力。用N-PC加FI注射辐照大鼠后24小时获得的PC显示I-E +细胞增加，而在用N-PC加FI注射辐照并骨髓重建的大鼠后24小时获得PC时，可以观察到I-A +细胞增加。这些发现似乎表明FI-PC2h和FI-PC24h在功能上是不同的，并且在注射RAG的FI后24小时获得的细胞群既不是来自注射RAG的FI后2小时存在的细胞群，也不是来自正常PC。它们似乎需要骨髓前体细胞。
