Ferro M E, Romero-Piffiguer M, Riera C M
Inmunologia, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
Eur J Immunol. 1990 May;20(5):1007-12. doi: 10.1002/eji.1830200509.
The present report describes the structural and functional characteristics of the population of peritoneal cells (PC) from rats injected intraperitoneally (i.p.) 2 h or 24 h previously with a suppressor dose of a purified fraction (FI) of rat male accessory glands (RAG: FI-PC2h and FI-PC24h, respectively). Both populations of PC showed the presence of the autoantigens of RAG on their membrane. The study of cell surface marker showed increase of OX17 (I-E) in FI-PC2h and OX6 (I-A) in FI-PC24h. I.p. injection of FI-PC2h 10 and 3 days prior to immunization with chemically modified (diazotized arsanilic and sulfanilic acid) RAG (MRAG) in complete Freund's adjuvant induced suppression of the delayed-type hypersensitivity (DTH) response to MRAG, whereas the i.p. injection of FI-PC24h induced potentiation of DTH response to MRAG when compared with controls (animals injected 10 and 3 days prior to immunization with PC from rats injected i.p. with an unrelated antigen). The treatment of FI-PC2h with OX17 and FI-PC24h with OX6 prior to the transfer blocked the suppression and potentiation described above. These findings show that the i.p. injection of an FI of RAG at a suppressor dose induces PC with immunologically opposite characteristics, depending on the time of obtention.
本报告描述了腹腔注射（i.p.）2小时或24小时前接受大鼠雄性附属性腺纯化组分（FI）抑制剂量（分别为FI-PC2h和FI-PC24h）的大鼠腹腔细胞（PC）群体的结构和功能特征。这两个PC群体的细胞膜上均存在RAG自身抗原。细胞表面标志物研究显示，FI-PC2h中OX17（I-E）增加，FI-PC24h中OX6（I-A）增加。在完全弗氏佐剂中用化学修饰（重氮化对氨基苯胂酸和对氨基苯磺酸）的RAG（MRAG）免疫前10天和3天腹腔注射FI-PC2h可诱导对MRAG的迟发型超敏反应（DTH）抑制，而与对照组（免疫前10天和3天腹腔注射腹腔注射无关抗原大鼠的PC的动物）相比，腹腔注射FI-PC24h可诱导对MRAG的DTH反应增强。在转移前用OX17处理FI-PC2h和用OX6处理FI-PC24h可阻断上述抑制和增强作用。这些发现表明，腹腔注射抑制剂量的RAG的FI可诱导具有免疫相反特征的PC，这取决于获取时间。
