Rosa e Silva Lívia Kmetzsch, Staats Charley Christian, Goulart Letícia Silveira, Morello Luis Gustavo, Pelegrinelli Fungaro Maria Helena, Schrank Augusto, Vainstein Marilene H
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
Res Microbiol. 2008 Apr;159(3):221-9. doi: 10.1016/j.resmic.2007.12.006. Epub 2008 Jan 3.
Cryptococcus neoformans is a basidiomycetous fungus and an opportunistic human pathogen that causes infections in both immunocompromised and immunocompetent hosts. The ability to survive and proliferate at the human body temperature is an essential virulence attribute of this microorganism. Representational difference analysis (RDA) was used to profile gene expression in C. neoformans grown at 37 degrees C or 25 degrees C. Contig assembly of 300 high-quality sequenced cDNAs and comparison analysis to the GenBank database led to the identification of transcripts that may be critical for both pathogen-host interactions and responses to either low or high temperature growth. Gene products involved in cell wall integrity, stress response, filamentation, oxidative metabolism, protein targeting and fatty acids metabolism were induced at 37 degrees C. In addition, genes related to chromatin silencing and phospholipid transport were upregulated at 25 degrees C. Therefore, our RDA analysis, comparing saprophytic and host temperature conditions, revealed new genes with potential involvement in C. neoformans virulence.
新型隐球菌是一种担子菌纲真菌，也是一种机会性人类病原体，可在免疫功能低下和免疫功能正常的宿主中引起感染。在人体温度下存活和增殖的能力是这种微生物的一种基本毒力属性。代表性差异分析（RDA）用于分析新型隐球菌在37℃或25℃下生长时的基因表达情况。对300个高质量测序cDNA进行重叠群组装，并与GenBank数据库进行比较分析，从而鉴定出可能对病原体与宿主相互作用以及对低温或高温生长的反应至关重要的转录本。参与细胞壁完整性、应激反应、丝状化、氧化代谢、蛋白质靶向和脂肪酸代谢的基因产物在37℃时被诱导表达。此外，与染色质沉默和磷脂转运相关的基因在25℃时上调。因此，我们通过RDA分析比较腐生和宿主温度条件，揭示了可能参与新型隐球菌毒力的新基因。
